Nurrachman Aga Satria, Azhari Azhari, Epsilawati Lusi, Pramanik Farina
Department of Oral and Maxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Padjadjaran University, Bandung, West Java, Indonesia.
Eur J Dent. 2023 May;17(2):283-295. doi: 10.1055/s-0042-1757466. Epub 2023 Jan 30.
Angiogenesis plays an important role in fracture healing with vascular endothelial growth factor (VEGF) as the main protein involved. Micro-computed tomography (CT) angiography may be used to analyze this revascularization with several parameters such as number of branches, total volume, and diameter. This systematic review is aimed to assess available studies on the temporal pattern of vascular imaging on micro-CT angiographs, especially in terms of the number of branches, total volume, and diameter as well as the temporal pattern of VEGF mRNA expression as the molecular comparison during bone fracture healing. This review was conducted according to the PRISMA guidelines. Electronic database searches were performed using PubMed, ProQuest, ScienceDirect, EBSCOhost, Taylor & Francis Online, and hand searching. The search strategy and keywords were adjusted to each database using the Boolean operators and other available limit functions to identify most relevant articles based on our inclusion and exclusion criteria. Screening and filtration were done in several stages by removing the duplicates and analyzing each title, abstract, and full-text in all included entries. Data extraction was done for syntheses to summarize the temporal pattern of each parameter. A total of 28 articles were eligible and met all criteria, 11 articles were synthesized in its angiograph's analysis, 16 articles were synthesized in its VEGF mRNA expression analysis, and 1 article had both parameters analyzed. The overall temporal pattern of both three micro-CT angiographic parameters and VEGF mRNA expression was in line qualitatively. The number of branches, total volume, and diameter of the blood vessels in micro-CT angiography showed an exponential rise at week 2 and decline at week 3 of fracture healing, with the VEGF mRNA expression concurrently showing a consistent pattern in the phase.
血管生成在骨折愈合中起重要作用，血管内皮生长因子（VEGF）是主要参与的蛋白质。微计算机断层扫描（CT）血管造影可用于分析这种血管再生情况，其参数包括分支数量、总体积和直径等。本系统评价旨在评估有关微CT血管造影上血管成像时间模式的现有研究，特别是在分支数量、总体积和直径方面，以及作为骨折愈合过程中分子对照的VEGF mRNA表达的时间模式。本评价按照PRISMA指南进行。使用PubMed、ProQuest、ScienceDirect、EBSCOhost、Taylor & Francis Online进行电子数据库检索，并进行手工检索。使用布尔运算符和其他可用的限制功能，根据每个数据库调整检索策略和关键词，以根据我们的纳入和排除标准识别最相关的文章。通过去除重复项并分析所有纳入条目的每个标题、摘要和全文，分几个阶段进行筛选和过滤。进行数据提取以进行综合分析，总结每个参数的时间模式。共有28篇文章符合所有标准，其中11篇文章在血管造影分析中进行了综合分析，16篇文章在VEGF mRNA表达分析中进行了综合分析，1篇文章对两个参数都进行了分析。三个微CT血管造影参数和VEGF mRNA表达的总体时间模式在定性上是一致的。微CT血管造影中血管的分支数量、总体积和直径在骨折愈合第2周呈指数上升，在第3周下降，VEGF mRNA表达在该阶段同时呈现一致的模式。
