Ashraf Suhail, Krishna Nayana R U, Nakkeeran Sevugapperumal, Nallusamy Saranya, Fatimah Nusrat, Jardan Yousef A Bin, Raish Mohammad
Chair of Genetics and Genomics, Faculty of Biology and Centre for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.
Department of Plant Biotechnology, Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur, Kerala, India.
J Basic Microbiol. 2025 Mar;65(3):e2400533. doi: 10.1002/jobm.202400533. Epub 2024 Dec 10.
Research on nematode management globally highlights the use of nematicidal biomolecules and biocontrol agents. However, the availability of biomolecules to manage plant-parasitic nematodes remains limited. The discovery of microbial biomolecules offers new opportunities in this field, though they are underexplored for suppressing nematodes. This study focused on identifying biomolecules from Bacillus licheniformis (MW301654) to manage Meloidogyne incognita, a root-knot nematode infecting banana. In silico protein-ligand interactions revealed that, Nicotinamide mononucleotide, produced during the ditrophic interaction of B. licheniformis (MW301654) with Fusarium oxysporum f. sp. cubense was effective against M. incognita protein targets including cytochrome C oxidase subunit 1, calreticulin, neuropeptide G-protein coupled receptor, chorismate mutase 1, venom allergen-like proteins and β-1,4-endoglucanase than the commercially used nematicides carbofuran 3G and fluensulfone. In vitro bioassays further validated nicotinamide mononucleotide nematicidal activity. At concentrations of 93, 76, and 69 ppm, nicotinamide mononucleotide caused 50% mortality of second-stage juveniles after 24, 48, and 72 h, respectively, while 213, 132, and 101 ppm resulted in 95% mortality. Egg hatching was also significantly reduced, with only 1% hatching at 150 ppm. The study emphasized the potential of Nicotinamide mononucleotide as a novel biopesticide for the management of M. incognita infection in banana.
全球关于线虫管理的研究突出了杀线虫生物分子和生物防治剂的使用。然而，用于管理植物寄生线虫的生物分子的可用性仍然有限。微生物生物分子的发现为该领域提供了新的机会，尽管它们在抑制线虫方面尚未得到充分探索。本研究的重点是从地衣芽孢杆菌（MW301654）中鉴定生物分子，以管理南方根结线虫，一种感染香蕉的根结线虫。计算机模拟蛋白质-配体相互作用表明，地衣芽孢杆菌（MW301654）与尖孢镰刀菌古巴专化型营养互作过程中产生的烟酰胺单核苷酸，比市售杀线虫剂克百威3G和氟吡菌酰胺对南方根结线虫的蛋白质靶点更有效，这些靶点包括细胞色素C氧化酶亚基1、钙网蛋白、神经肽G蛋白偶联受体、分支酸变位酶1、毒液过敏原样蛋白和β-1,4-内切葡聚糖酶。体外生物测定进一步验证了烟酰胺单核苷酸的杀线虫活性。在浓度为93、76和69 ppm时，烟酰胺单核苷酸分别在24、48和72小时后导致第二代幼虫50%的死亡率，而213、132和101 ppm则导致95%的死亡率。卵孵化也显著减少，在150 ppm时只有1%孵化。该研究强调了烟酰胺单核苷酸作为一种新型生物农药用于管理香蕉南方根结线虫感染的潜力。
