Shovon Shahriar Rahman, Uematsu Tomoki, Osaki Yuki, Masui Tatsushi, Koyama Takashi, Fujiwara Toshinobu, Ushida Chisato
The United Graduate School of Agricultural Sciences, Iwate University, Morioka, 020-8550, Japan.
Graduate School of Agriculture and Life Science, Hiorosaki University, Hirosaki, 036-8561, Japan.
Biochem Biophys Res Commun. 2025 Jan;742:151125. doi: 10.1016/j.bbrc.2024.151125. Epub 2024 Dec 9.
The precursor rRNA (pre-rRNA) processing pathway in Caenorhabditis elegans remains unidentified. We have determined the cleavage site within the internal transcribed spacer 2, which generates pre-rRNA f of 5.8S rRNA and pre-rRNA g of 26S rRNA. Ribosome biogenesis is the most energetically demanding process of the cell. Under starved conditions, cells conserve energy by reconfiguring ribosome biogenesis to sustain proper growth. We investigated the short-term effects of starvation on the ribosomal RNA (rRNA) maturation in C. elegans. Northern blot analysis revealed significant changes in both small and large pre-rRNAs, with a substantial decrease in pre-rRNA c'1/27SA of the large subunit and an increase in pre-rRNA d of the small subunit. Additionally, most pre-rRNAs accumulated in the nucleolar center, while the rRNA maturation factor FIB-1 accumulated at the nucleolar periphery. Nutritional replenishment restored the pre-rRNA accumulation pattern and the distribution of pre-rRNAs in the nucleolus to pre-starvation conditions.
秀丽隐杆线虫中的前体核糖体RNA（pre-rRNA）加工途径仍未明确。我们已经确定了内部转录间隔区2内的切割位点，该位点产生5.8S rRNA的pre-rRNA f和26S rRNA的pre-rRNA g。核糖体生物合成是细胞中能量需求最高的过程。在饥饿条件下，细胞通过重新配置核糖体生物合成来保存能量，以维持正常生长。我们研究了饥饿对秀丽隐杆线虫核糖体RNA（rRNA）成熟的短期影响。Northern印迹分析显示，大小前体rRNA均有显著变化，大亚基的pre-rRNA c'1/27SA大幅减少，小亚基的pre-rRNA d增加。此外，大多数前体rRNA积聚在核仁中心，而rRNA成熟因子FIB-1积聚在核仁周边。营养补充使前体rRNA的积累模式和核仁中前体rRNA的分布恢复到饥饿前的状态。
