Hokii Yusuke, Sasano Yumi, Sato Mayu, Sakamoto Hiroshi, Sakata Kazumi, Shingai Ryuzo, Taneda Akito, Oka Shigenori, Himeno Hyouta, Muto Akira, Fujiwara Toshinobu, Ushida Chisato
Functional Genomics and Technology, United Graduate School of Agricultural Science, Iwate University, 18-8 Ueda 3-chome, Morioka 020-8550.
Nucleic Acids Res. 2010 Sep;38(17):5909-18. doi: 10.1093/nar/gkq335. Epub 2010 May 11.
CeR-2 RNA is one of the newly identified Caenorhabditis elegans noncoding RNAs (ncRNAs). The characterization of CeR-2 by RNomic studies has failed to classify it into any known ncRNA family. In this study, we examined the spatiotemporal expression patterns of CeR-2 to gain insight into its function. CeR-2 is expressed in most cells from the early embryo to adult stages. The subcellular localization of this RNA is analogous to that of fibrillarin, a major protein of the nucleolus. It was observed that knockdown of C/D small nucleolar ribonucleoproteins (snoRNPs), but not of H/ACA snoRNPs, resulted in the aberrant nucleolar localization of CeR-2 RNA. A mutant worm with a reduced amount of cellular CeR-2 RNA showed changes in its pre-rRNA processing pattern compared with that of the wild-type strain N2. These results suggest that CeR-2 RNA is a C/D snoRNA involved in the processing of rRNAs.
CeR-2 RNA是新鉴定出的秀丽隐杆线虫非编码RNA（ncRNA）之一。通过RNA组学研究对CeR-2进行的特征描述未能将其归类到任何已知的ncRNA家族中。在本研究中，我们检测了CeR-2的时空表达模式，以深入了解其功能。CeR-2在从早期胚胎到成虫阶段的大多数细胞中都有表达。这种RNA的亚细胞定位与核仁的主要蛋白质纤维蛋白原的定位相似。据观察，敲低C/D小核仁核糖核蛋白（snoRNP），而不是H/ACA snoRNP，会导致CeR-2 RNA在核仁中的异常定位。与野生型菌株N2相比，细胞内CeR-2 RNA含量减少的突变线虫在其前体rRNA加工模式上出现了变化。这些结果表明CeR-2 RNA是一种参与rRNA加工的C/D snoRNA。
