Dong Wenbo, Fu Rongxin, Zhang Nan, Zhao Jing, Ma Yudan, Cui Han, Zhang Jiangjiang, Zhao Zipeng, Li Hang, Zhao Yunxia, Lu Yao, Chen Zhizhong, Xu Tianming, Xie Huikai, Yu Qian, Zhang Shuailong
Beijing Advanced Innovation Center for Intelligent Robots and Systems, School of Mechanical Engineering, Beijing Institute of Technology, Beijing, 100081, PR China.
School of Medical Technology, Beijing Institute of Technology, Beijing, 100081, PR China; Chongqing Institute of Microelectronics and Microsystems, Beijing Institute of Technology, Beijing, 100081, PR China.
Biosens Bioelectron. 2025 Mar 1;271:117036. doi: 10.1016/j.bios.2024.117036. Epub 2024 Dec 4.
This study introduces an advanced bioanalytical platform that combines digital microfluidics (DMF) with Raman spectroscopy, effectively addressing common issues in bioanalysis such as sample contamination, excessive consumption of samples and reagents, and manual handling. Our innovative system is engineered to handle diverse sample types and enables both sample preparation and in-situ analysis on a single device, utilizing less than 5 μL of samples and reagents. It incorporates a Translucent Raman Enhancement Stack (TRES) sensor, which boosts the detection signal, and includes droplet-driving functionality for automated processing of complex samples in a compact setting. The hydrophilic surface of the TRES sensor draws analytes into the detection zone, expediting sample attachment and enhancing the surface-enhanced Raman scattering (SERS). Moreover, the TRES sensors are cost-effective, straightforward to manufacture, and scalable, making them suitable for widespread production and single-use bioanalytical applications. This integrated and automated approach streamlines the sample preparation and analysis processes, enhances detection efficiency, and achieves high sensitivity, excellent linearity, and effective detection of different biochemical analytes. We validate the system's accuracy through on-device enrichment and analysis of exosomes from serum, demonstrating its capability for real-time, on-site analysis of complex biological samples.
本研究介绍了一种先进的生物分析平台，该平台将数字微流控（DMF）与拉曼光谱相结合，有效解决了生物分析中的常见问题，如样品污染、样品和试剂消耗过多以及人工操作等。我们的创新系统经过精心设计，可处理多种样品类型，并能在单个设备上实现样品制备和原位分析，样品和试剂的用量不到5微升。它集成了一个半透明拉曼增强堆栈（TRES）传感器，可增强检测信号，并具备液滴驱动功能，能在紧凑的环境中对复杂样品进行自动化处理。TRES传感器的亲水性表面将分析物吸引到检测区域，加快样品附着并增强表面增强拉曼散射（SERS）。此外，TRES传感器具有成本效益、制造简单且可扩展，适用于大规模生产和一次性生物分析应用。这种集成的自动化方法简化了样品制备和分析过程，提高了检测效率，并实现了高灵敏度、出色的线性度以及对不同生化分析物的有效检测。我们通过对血清中外泌体进行设备上的富集和分析来验证系统的准确性，证明了其对复杂生物样品进行实时、现场分析的能力。
