Li Dongshuo, Hu Hanqi, Zhang Hanzhi, Shang Lei, Zhao Tao, Zhao Qingchen, Zhang Shuhao, Ma Fucun, Liang Guowei, Fu Rongxin, Liu Xuekai
Department of Clinical Laboratory, Aerospace Center Hospital, Beijing 100049, China.
School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.
Micromachines (Basel). 2025 Jun 30;16(7):785. doi: 10.3390/mi16070785.
Antithrombin (AT) plays a crucial role in the human anticoagulant system and has extensive clinical applications. However, traditional detection methods often require large sample volumes, complex procedures, and lengthy processing times.
We integrated digital microfluidics technology with AT detection to develop a point-of-care testing (POCT) device that is user-friendly and fully automated for real-time AT testing.
This device allows for automation and enhanced adaptability to various settings, requiring only a minimal sample volume (whole capillary blood), thereby omitting steps such as plasma separation to save time and improve clinical testing efficiency. Comparisons with conventional AT activity detection methods demonstrate a high degree of consistency in the results obtained with this device.
The AT detection system we developed exhibits significant effectiveness and holds substantial research potential, positioning it to evolve into a clinically impactful POCT solution for AT assessment.
抗凝血酶（AT）在人体抗凝系统中起关键作用，具有广泛的临床应用。然而，传统检测方法通常需要大量样本、复杂的程序以及较长的处理时间。
我们将数字微流控技术与AT检测相结合，开发出一种即时检测（POCT）设备，该设备用户友好且完全自动化，可进行实时AT检测。
该设备实现了自动化，并增强了对各种环境的适应性，仅需极少的样本量（全毛细血管血），从而省略了血浆分离等步骤，节省了时间并提高了临床检测效率。与传统AT活性检测方法的比较表明，该设备所获结果具有高度一致性。
我们开发的AT检测系统显示出显著的有效性，并具有巨大的研究潜力，有望发展成为用于AT评估的具有临床影响力的POCT解决方案。
