Gómez-Galdós Celia, Perez-Asensio Andrea, Fernández-Manteca María Gabriela, García García Borja, Algorri José Francisco, López-Higuera José Miguel, Rodríguez-Cobo Luis, Cobo Adolfo
Photonics Engineering Group, Universidad de Cantabria, 39005 Santander, Spain.
Instituto de Investigación Sanitaria Valdecilla (IDIVAL), 39011 Santander, Spain.
Biosensors (Basel). 2025 Mar 6;15(3):172. doi: 10.3390/bios15030172.
Water testing is becoming increasingly important due to dangerous phenomena such as Harmful Algal Blooms (HABs). Commonly, the content of a water sample is measured for the detection, monitoring and control of these events. Raman spectroscopy is a technique for the molecular characterization of materials in solid, liquid or gaseous form, which makes it an attractive method for analysing materials' components. However, Raman scattering is a weak optical process and requires an accurate system for detection. In our work, we present, from design to fabrication, a microfluidic device on fused silica adapted to optimise the Raman spectrum of liquid samples when using a Raman probe. The device features a portable design for rapid on-site continuous flow measurements avoiding the use of large, costly and complex laboratory equipment. The main manufacturing technique used was ultrafast laser-assisted etching (ULAE). Finally, the effectiveness of the microfluidic device was demonstrated by comparing the Raman spectra of a known species of cyanobacteria with those obtained using other conventional substrates in laboratory analysis. The results demonstrate that the microfluidic device, under continuous flow conditions, exhibited a lower standard deviation of the Raman signal, reduced background noise and avoided signal variations caused by sample drying in static measurements.
由于有害藻华（HABs）等危险现象，水质检测变得越来越重要。通常，通过测量水样的成分来检测、监测和控制这些事件。拉曼光谱是一种用于对固态、液态或气态材料进行分子表征的技术，这使其成为分析材料成分的一种有吸引力的方法。然而，拉曼散射是一个微弱的光学过程，需要精确的检测系统。在我们的工作中，我们展示了一种从设计到制造的微流控装置，该装置基于熔融石英，在使用拉曼探头时能够优化液体样品的拉曼光谱。该装置采用便携式设计，可进行快速现场连续流动测量，无需使用大型、昂贵且复杂的实验室设备。主要制造技术是超快激光辅助蚀刻（ULAE）。最后，通过将已知蓝藻物种的拉曼光谱与实验室分析中使用其他传统基质获得的光谱进行比较，证明了微流控装置的有效性。结果表明，在连续流动条件下，该微流控装置的拉曼信号标准偏差更低，背景噪声降低，并且避免了静态测量中样品干燥引起的信号变化。
