Almehmadi Mazen, Allahyani Mamdouh, Aljuaid Abdulelah, Alsuwat Meshari A, Halawi Mustafa
From the Department of Clinical Laboratory Sciences (Almehmadi, Allahyani, Aljuaid, Alsuwat), College of Applied Medical Sciences, Taif University, Taif; and from the College of Nursing and Health Sciences (Halawi), Jazan University, Jazan, Kingdom of Saudi Arabia.
Saudi Med J. 2024 Dec;45(12):1312-1317. doi: 10.15537/smj.2024.45.12.20240498.
To investigate the numbers of natural killer (NK) cells, CD56+ T-cells and CD56- T cells, and the levels ofCD16 in healthy individuals seropositive for herpes simplex virus Type 1 (HSV-1). Specifically, it seeks to measure the levels of these cells to learn about the possible immunological significance during HSV-1 seropositive status.
This study employed a cross-sectional research design to examine the levels of CD56+ T-cells and CD16+ among individuals seropositive for herpes simplex virus type 1 (HSV-1) in Taif city. A total of 112 participants were enrolled, with HSV-1 serostatus determined via ELISA, and cellular evaluation conducted using flow cytometry. The study was performed between January 2023 to July 2023.
Our study found 36% HSV-1 seropositivity, contrasting with higher rates in Saudi Arabia. No significant age or gender differences were observed. HSV-1 seropositive individuals showed elevated dim CD56 NK cells and CD56+ T-cells, aligning with prior research on lymphocyte infiltration during HSV-1 activation. Further investigation is warranted for CD56- T-cells and bright NK cells.
HSV-1 seropositive individuals showed elevated dim CD56 NK cells and CD56+ T-cells, consistent with lymphocyte activities during viral activation. CD16 expression on CD56+ T-cells suggests their involvement in viral defence, emphasizing the need for further investigation into immune responses against HSV-1.
研究单纯疱疹病毒1型（HSV-1）血清学阳性的健康个体中自然杀伤（NK）细胞、CD56⁺ T细胞和CD56⁻ T细胞的数量以及CD16的水平。具体而言，旨在测量这些细胞的水平，以了解HSV-1血清学阳性状态期间可能的免疫学意义。
本研究采用横断面研究设计，检测塔伊夫市单纯疱疹病毒1型（HSV-1）血清学阳性个体中CD56⁺ T细胞和CD16⁺的水平。共招募了112名参与者，通过酶联免疫吸附测定（ELISA）确定HSV-1血清状态，并使用流式细胞术进行细胞评估。该研究于2023年1月至2023年7月进行。
我们的研究发现HSV-1血清阳性率为36%，与沙特阿拉伯的较高比率形成对比。未观察到显著的年龄或性别差异。HSV-1血清学阳性个体显示低表达CD56自然杀伤细胞和CD56⁺ T细胞升高，这与之前关于HSV-1激活期间淋巴细胞浸润的研究一致。对于CD56⁻ T细胞和高表达自然杀伤细胞，有必要进一步研究。
HSV-1血清学阳性个体显示低表达CD56自然杀伤细胞和CD56⁺ T细胞升高，这与病毒激活期间的淋巴细胞活动一致。CD56⁺ T细胞上的CD16表达表明它们参与病毒防御，强调需要进一步研究针对HSV-1的免疫反应。
