Li Runjing, Qiu Jiehua, Shi Huanbin, Jiang Nan, Lan Bo, Kou Yanjun
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
Institute of Plant Protection, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China.
Plant Dis. 2025 Jun;109(6):1211-1217. doi: 10.1094/PDIS-04-24-0926-SR. Epub 2025 Jun 2.
The avirulence () genes of are pivotal in eliciting resistance responses in rice, which are mediated by resistance () genes in rice. Monitoring the variation of genes in the pathogen is essential for strategically deploying genes in rice cultivation regions. In this study, a total of 214 isolates were collected from Jiangxi Province, China, in 2022, and the distribution and variation of genes in these isolates were analyzed by PCR amplification and sequencing. The results indicated that , , , and loci were detected in more than 95% of isolates. In addition, the and loci were found in 84.1 and 0.9% of isolates, respectively. Notably, none of the isolates contained the gene. Sequencing results revealed various types of variants, including base replacement, deletion, and insertion, in , , and loci. Moreover, a transposon insertion was identified in the promoter region of . Furthermore, variant isolates were then utilized to inoculate the rice monogenic lines IRBLta-K1, IRBLkm-Ts, IRBLKh-K3, and IRBLb-B. The results demonstrated that the rice monogenic line carrying or was unable to recognize the isolates with the variant or . However, the rice monogenic line with or remained capable of recognizing the isolates with alleles in Jiangxi Province. The information obtained from this study is valuable for breeding blast-resistant rice varieties that will be cultivated in Jiangxi Province, China.
稻瘟病菌的无毒（）基因在引发水稻的抗性反应中起关键作用，这些反应由水稻中的抗性（）基因介导。监测病原菌中基因的变异对于在水稻种植区战略性地部署基因至关重要。在本研究中，2022年从中国江西省共收集了214个分离株，并通过PCR扩增和测序分析了这些分离株中基因的分布和变异。结果表明，超过95%的分离株中检测到、、和位点。此外，分别在84.1%和0.9%的分离株中发现了和位点。值得注意的是，所有分离株均未含有基因。测序结果揭示了、和位点中存在多种类型的变异，包括碱基替换、缺失和插入。此外，在的启动子区域鉴定到一个转座子插入。进而利用变异分离株接种水稻单基因系IRBLta-K1、IRBLkm-Ts、IRBLKh-K3和IRBLb-B。结果表明，携带或的水稻单基因系无法识别具有变异或的分离株。然而，具有或的水稻单基因系仍能够识别江西省具有等位基因的分离株。本研究获得的信息对于在中国江西省培育抗稻瘟病水稻品种具有重要价值。
