Mansouritorghabeh H, Monard A, Heubel-Moenen F, Leentjens J, Stroobants A, Henskens Y
Central Diagnostic Laboratories, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Internal Medicine, Division of Thrombosis and Hemostasis, Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands.
Int J Lab Hematol. 2025 Apr;47(2):201-211. doi: 10.1111/ijlh.14403. Epub 2024 Dec 10.
A wide variety of laboratory hemostasis tests is available, but the majority is plasma-based, static and unable to assess platelet function and fibrin formation simultaneously. The Total Thrombus-Formation Analysis System (T-TAS) is a microchip-based flow chamber system that simulates in vivo conditions for evaluating whole blood thrombogenicity.
A comprehensive overview of its applicability in different thrombosis and hemostasis related clinical situations is lacking and therefore this scoping review was performed.
MATERIALS & METHODS: A literature search was done using the electronic databases PubMed, Scopus and Embase on January 7, 2024. Original studies assessing the usefulness of the T-TAS in thrombosis and hemostasis related clinical situations were eligible for this scoping review.
A total of 28 studies were included; six studies investigating the role of the T-TAS in congenital bleeding disorders, five studies using the T-TAS to assess 1-year bleeding risk in patients on antiplatelet or anticoagulant medications, four studies investigating the effects of thrombocytopenia and hemodialysis on thrombus formation as measured by the T-TAS, 11 studies testing the applicability of the T-TAS in the monitoring of anticoagulant and antiplatelet therapies and eventually two studies on the ability of the T-TAS to assess the thrombogenicity in different disease entities.
DISCUSSION & CONCLUSION: The T-TAS method is an interesting technology that mimics the complex biological coagulation process using shear forces, creating a "blood vessel component on a chip". More research is needed, but it could eventually function as a screening test for platelet function and coagulation. Moreover, it could be used to detect the presence of anticoagulant and/or antiplatelet medication.
有多种实验室止血测试方法可供使用，但大多数基于血浆，是静态的，无法同时评估血小板功能和纤维蛋白形成。全血栓形成分析系统（T-TAS）是一种基于微芯片的流动腔系统，可模拟体内条件以评估全血的血栓形成能力。
目前缺乏对其在不同血栓形成和止血相关临床情况中适用性的全面概述，因此进行了这项范围综述。
于2024年1月7日使用电子数据库PubMed、Scopus和Embase进行文献检索。评估T-TAS在血栓形成和止血相关临床情况中有用性的原始研究符合本范围综述的要求。
共纳入28项研究；六项研究调查了T-TAS在先天性出血性疾病中的作用，五项研究使用T-TAS评估抗血小板或抗凝药物治疗患者的1年出血风险，四项研究调查血小板减少症和血液透析对T-TAS测量的血栓形成的影响，十一项研究测试了T-TAS在监测抗凝和抗血小板治疗中的适用性，最后两项研究关于T-TAS评估不同疾病实体中血栓形成能力。
T-TAS方法是一项有趣的技术，它利用剪切力模拟复杂的生物凝血过程，创造了一个“芯片上的血管组件”。还需要更多研究，但它最终可能作为血小板功能和凝血的筛查测试。此外，它可用于检测抗凝和/或抗血小板药物的存在。
