Abadikhah Marie, Persson Frank, Farewell Anne, Wilén Britt-Marie, Modin Oskar
Division of Water Environment Technology, Department of Architecture and Civil Engineering, Chalmers University of Technology, Sven Hultins gata 6, SE-412 96 Gothenburg, Sweden.
Department of Chemistry and Molecular Biology, University of Gothenburg, SE-405 30 Gothenburg, Sweden.
ISME Commun. 2024 Nov 15;4(1):ycae143. doi: 10.1093/ismeco/ycae143. eCollection 2024 Jan.
In microbial electrolysis cells (MECs), microbial communities catalyze conversions between dissolved organic compounds, electrical energy, and energy carriers such as hydrogen and methane. Bacteria and archaea, which catalyze reactions on the anode and cathode of MECs, interact with phages; however, phage communities have previously not been examined in MECs. In this study, we used metagenomic sequencing to study prokaryotes and phages in nine MECs. A total of 852 prokaryotic draft genomes representing 278 species, and 1476 phage contigs representing 873 phage species were assembled. Among high quality prokaryotic genomes (>95% completion), 55% carried a prophage, and the three spp. that dominated the anode communities all carried prophages. , one of the bacteria dominating the anode communities, carried a CRISPR spacer showing evidence of a previous infection by a phage present in the liquid of some MECs. spp. and an sp., which dominated the cathodes, had several associations with spp. The results of this study show that phage communities in MECs are diverse and interact with functional microorganisms on both the anode and cathode.
在微生物电解池（MECs）中，微生物群落催化溶解有机化合物、电能以及诸如氢气和甲烷等能量载体之间的转化。在MECs的阳极和阴极催化反应的细菌和古菌与噬菌体相互作用；然而，此前尚未对MECs中的噬菌体群落进行过研究。在本研究中，我们使用宏基因组测序来研究9个MECs中的原核生物和噬菌体。共组装出代表278个物种的852个原核生物草图基因组，以及代表873个噬菌体物种的1476个噬菌体重叠群。在高质量原核生物基因组（完成度>95%）中，55%携带原噬菌体，并且在阳极群落中占主导地位的3个物种均携带原噬菌体。在阳极群落中占主导地位的细菌之一，携带一个CRISPR间隔序列，显示出曾被某些MECs液体中存在的一种噬菌体感染的证据。在阴极占主导地位的物种和物种与物种有几种关联。本研究结果表明，MECs中的噬菌体群落具有多样性，并与阳极和阴极上的功能微生物相互作用。
