Sheikholeslami Seyed Alireza, Esmaeili Javad, Jalise Saeedeh Zare, Barati Aboulfazl
Department of Chemical Engineering, Faculty of Engineering, Arak University, Arak, 38156-88349, Iran.
Department of Tissue Engineering, TISSUEHUB Co., Tehran, Iran.
Heliyon. 2024 Nov 23;10(23):e40670. doi: 10.1016/j.heliyon.2024.e40670. eCollection 2024 Dec 15.
This study aimed to develop an innovative, intelligent wound dressing capable of signaling infections through color changes.
DESIGN/METHODOLOGY/APPROACH: Using response surface methodology, the Rhodamine B fluorescence colorant was encapsulated within colloidal nanoparticles and integrated into a sodium alginate patch at various concentrations. The physical and chemical characteristics of the nanoparticles and the wound dressing were thoroughly analyzed via dynamic light scattering (DLS), zeta potential measurements, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). Additionally, the biodegradability, hydrophilicity, swelling behavior, release kinetics, porosity, mechanical properties, biocompatibility, and infection detection capability of the wound dressing were evaluated.
The results indicated that the average diameter of the synthesized colloidal nanoparticles was 300 nm before loading with Rhodamine B and increased to 400 nm after loading, with zeta potentials of 52 mV and -6 mV, respectively. The Rhodamine B-loaded wound dressing demonstrated adequate levels of swelling and hydrophilicity. Release studies revealed the gradual release of Rhodamine B at low pH. Cytotoxicity assays confirmed the high biocompatibility of the engineered wound dressing with the L929 cell line. Furthermore, bacterial exposure experiments indicated that the color change was activated in the presence of infection, making it visible under UV-A light.
ORIGINALITY/VALUE: This research presents a novel approach to wound care by developing a smart wound dressing that can detect infections via color changes. These findings underscore the potential of this innovative wound dressing to improve infection management in clinical settings through its responsive and biocompatible design.
本研究旨在开发一种创新的智能伤口敷料，能够通过颜色变化来指示感染情况。
设计/方法/途径：采用响应面法，将罗丹明B荧光着色剂封装在胶体纳米颗粒中，并以不同浓度整合到海藻酸钠贴片中。通过动态光散射（DLS）、zeta电位测量、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）对纳米颗粒和伤口敷料的物理和化学特性进行了全面分析。此外，还评估了伤口敷料的生物降解性、亲水性、膨胀行为、释放动力学、孔隙率、机械性能、生物相容性和感染检测能力。
结果表明，合成的胶体纳米颗粒在负载罗丹明B之前的平均直径为300nm，负载后增加到400nm，zeta电位分别为52mV和-6mV。负载罗丹明B的伤口敷料表现出足够的膨胀性和亲水性。释放研究表明，在低pH值下罗丹明B会逐渐释放。细胞毒性试验证实了工程化伤口敷料与L929细胞系具有高度生物相容性。此外，细菌暴露实验表明，在感染存在时颜色变化被激活，在紫外-A光下可见。
原创性/价值：本研究通过开发一种能够通过颜色变化检测感染的智能伤口敷料，为伤口护理提供了一种新方法。这些发现强调了这种创新伤口敷料通过其响应性和生物相容性设计在临床环境中改善感染管理的潜力。
