Delabie Willem, De Bleser Dominique, Vandewalle Vicky, De Prest Marie-Laurence, Vandekerckhove Philippe, Compernolle Veerle, Feys Hendrik B
Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium.
Transfusion Innovation Center, Belgian Red Cross Flanders, Ghent, Belgium.
Curr Stem Cell Res Ther. 2025;20(2):218-227. doi: 10.2174/011574888X307274240610113314.
Human Platelet Lysate (hPL) is a platelet-derived and growth factor-rich supplement for cell culture. It can be prepared from surplus platelet concentrates initially intended for transfusion. Amotosalen-based photochemical pathogen inactivation of platelet concentrates is used in a number of blood establishments worldwide to minimize the risk of pathogen transmission from donor to patient.
This pathogen inactivation method has not been formally validated for direct use on hPL. Here, we have studied the impact of pathogen inactivation on hPL and compared it to untreated hPL prepared from pathogen-inactivated platelet concentrates or control hPL. We used mass spectrometry, ELISA, and mesenchymal stem cell culture for determining residual amotosalen, final growth factor content, and cell doubling, respectively.
The data have shown amotosalen concentrations to be reduced a thousand-fold following pathogen inactivation, leaving trace quantities of photosensitizer molecules in the final hPL product. Some growth factors have been reported to be significantly more impacted in hPL that is directly pathogen-inactivated compared to both control conditions. This has no significant effect on the growth kinetics of adipose-derived mesenchymal stem cells.
Direct amotosalen-based pathogen inactivation has a measurable impact on certain growth factors in hPL, but this does not outweigh the likely benefits of reducing the odds of donor-to-patient pathogen transmission.
人血小板裂解物（hPL）是一种用于细胞培养的血小板衍生且富含生长因子的补充剂。它可从最初用于输血的剩余血小板浓缩物中制备。基于氨甲环酸的血小板浓缩物光化学病原体灭活在全球许多血液机构中使用，以尽量减少病原体从供体传播给患者的风险。
这种病原体灭活方法尚未在hPL上进行直接验证。在此，我们研究了病原体灭活对hPL的影响，并将其与由病原体灭活的血小板浓缩物制备的未处理hPL或对照hPL进行比较。我们分别使用质谱分析、酶联免疫吸附测定和间充质干细胞培养来测定残留的氨甲环酸、最终生长因子含量和细胞倍增情况。
数据显示，病原体灭活后氨甲环酸浓度降低了一千倍，最终hPL产品中仅留下痕量的光敏剂分子。据报道，与两种对照条件相比，直接进行病原体灭活的hPL中的一些生长因子受到的影响更大。这对脂肪来源的间充质干细胞的生长动力学没有显著影响。
基于氨甲环酸的直接病原体灭活对hPL中的某些生长因子有可测量的影响，但这并不超过降低病原体从供体传播给患者几率的潜在益处。
