[肾小球免疫病理学：免疫荧光与免疫过氧化物酶的比较研究（作者译）]
[Glomerular immunopathology: comparative study with immunofluorescence and immunoperoxidase (author's transl)].
作者信息
Vieira L M, Sarno E N, Ruzany F, De Lacerda P R
出版信息
Rev Bras Pesqui Med Biol. 1979 Dec;12(6):377-81.
In order to evaluate the reliability of immunoperoxidase technic (IP) for immuno-deposits in frozen renal tissue, a single blind study was done comparing it with immunofluorescent technic (IF). Fifty renal biopsies were studied. Ten there were no deposits by IF or IP. In 40 of them the results showed the same specificity and greater intensity for IP. There were 25 cases with IgG, 22 with IgM, 9 with IgA, 3 with Clq, 27 with C3 and 3 with HbsAg. We concluded that IP can be used in place of IF for detection of the immune deposits in renal biopsies and that it offers an easier observation than the later.
为了评估免疫过氧化物酶技术（IP）用于检测冷冻肾组织中免疫沉积物的可靠性，进行了一项单盲研究，将其与免疫荧光技术（IF）进行比较。研究了50例肾活检标本。其中10例经IF或IP检测均无沉积物。在另外40例中，结果显示IP具有相同的特异性且强度更高。有25例IgG沉积，22例IgM沉积，9例IgA沉积，3例C1q沉积，27例C3沉积和3例HBsAg沉积。我们得出结论，IP可用于替代IF检测肾活检中的免疫沉积物，并且比后者观察起来更容易。
Zotero 插件