Chen Yan, Kou Xingpeng, Lian Wenjun, Hua Jiawen, Wang Yiqing, Chen Yang, Wang Qiao, Chai Guohua, Bai Yue
Forestry College, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Hohhot, 010018, People's Republic of China.
College of Landscape Architecture and Forestry, Qingdao Agricultural University, No. 700, Changcheng Road, Qingdao, 266109, People's Republic of China.
Plant Cell Rep. 2024 Dec 11;44(1):3. doi: 10.1007/s00299-024-03396-4.
Identification of salt-responsive calcineurin B-like protein-interacting protein kinases (CIPKs) in Populus. Calcineurin B-like protein-interacting protein kinases (CIPKs) play vital roles in plant growth and abiotic stress responses. Currently, the regulatory mechanisms underlying these processes mediated by CIPK proteins are not completely understood in woody species. This study provided the first systematic analysis of 31 Populus CIPK genes and investigated their evolutionary relationships, gene structures, motif compositions, and salt stress responses. A total of 11 pairs of paralogous PtCIPK genes were identified, of which three pairs may be resulted from whole genome duplication, and two pairs that may be created by tandem duplications. RT-qPCR analysis revealed that 93.5% (29/31) genes showed altered expression levels in roots after salt treatment. Ectopic expression of PdCIPK21 or PdCIPK31 in Arabidopsis resulted in significant increases of seed germination, root elongation and fresh weight under salt stress conditions. Cytological observation revealed that PdCIPK21/31 overexpression lines showed increased number, lumen area and cell wall thickness of xylem vessels, and higher lignin content in stems compared with the wild type, with decreased sensitivity to long-term salt stress treatment. Our results suggest that PdCIPK21/31 serve as candidate genes for improving wood production and enhancing salt tolerance of tree species.
杨树中盐响应性类钙调神经磷酸酶B互作蛋白激酶（CIPKs）的鉴定。类钙调神经磷酸酶B互作蛋白激酶（CIPKs）在植物生长和非生物胁迫响应中发挥着至关重要的作用。目前，在木本植物中，由CIPK蛋白介导的这些过程的调控机制尚未完全清楚。本研究首次对31个杨树CIPK基因进行了系统分析，并研究了它们的进化关系、基因结构、基序组成和盐胁迫响应。共鉴定出11对旁系同源PtCIPK基因，其中3对可能源于全基因组复制，2对可能由串联重复产生。RT-qPCR分析表明，93.5%（29/31）的基因在盐处理后根中的表达水平发生了变化。在拟南芥中异位表达PdCIPK21或PdCIPK31导致在盐胁迫条件下种子萌发、根伸长和鲜重显著增加。细胞学观察表明，与野生型相比，PdCIPK21/31过表达系木质部导管的数量、管腔面积和细胞壁厚度增加，茎中木质素含量更高，对长期盐胁迫处理的敏感性降低。我们的结果表明，PdCIPK21/31可作为改善木材产量和增强树种耐盐性的候选基因。
