Ertik Onur, Koroglu Pınar, Magaji Umar Faruk, Bulan Nihal Omur, Sacan Ozlem, Yanardag Refiye
Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpaşa, Avcilar, Istanbul, Türkiye.
Department of Chemistry, Faculty of Engineering and Science, Bursa Technical University, Yildirim, Bursa, Türkiye.
J Mol Histol. 2024 Dec 11;56(1):37. doi: 10.1007/s10735-024-10277-3.
Moringa oleifera (M. oleifera) is a popular medicinal plant that has become a wide research area in recent years due to its detected biological effects and its bioactive compounds. Valproic acid (VPA) is a medication used in the treatment of epilepsy and bipolar disorder and high doses or prolonged use of VPA can result in oxidative stress in cells. Since M. oleifera has high biological activities and contains many bioactive compounds, it is necessary to understand whether it plays a role in reducing oxidative damage, especially that caused VPA. The relationship between VPA and tongue tissue needs to be investigated, since VPA has negative effects on oral health and it is known that tongue tissue plays an important role in the continuity of oral health. In the present study, 3.0-3.5 month-old female Sprague Dawley rats (160-250 g) were divided into four groups (Control, Moringa, VPA, VPA + M), and VPA was administered via gavage. The aim was to understand the protective/preventive effects of ethanolic M. oleifera leaves extract against oxidative stress through biochemical parameters. Additionally, molecular docking studies were conducted on niazicin-A, niazimin-A, and niazimin-B found in M. oleifera leaves based on in vivo results. The results indicate that M. oleifera extract treats oxidative damage to the tongue tissue, and niazimin-A and niazimin-B particularly show high binding affinities to myeloperoxidase (MPO) and lactate dehydrogenase (LDH) enzymes. Further studies may suggest that the use of M. oleifera leaves extract with VPA could prevent potential negative effects on tongue tissue.
辣木（Moringa oleifera）是一种广受欢迎的药用植物，由于其已被检测到的生物学效应及其生物活性化合物，近年来已成为一个广泛的研究领域。丙戊酸（VPA）是一种用于治疗癫痫和双相情感障碍的药物，高剂量或长期使用VPA会导致细胞内氧化应激。由于辣木有很高的生物活性且含有许多生物活性化合物，因此有必要了解它是否在减少氧化损伤，尤其是由VPA引起的氧化损伤方面发挥作用。由于VPA对口腔健康有负面影响，且已知舌组织在口腔健康的维持中起重要作用，因此需要研究VPA与舌组织之间的关系。在本研究中，将3.0 - 3.5月龄的雌性斯普拉格-道利大鼠（160 - 250克）分为四组（对照组、辣木组、VPA组、VPA + 辣木组），通过灌胃给予VPA。目的是通过生化参数了解辣木叶乙醇提取物对氧化应激的保护/预防作用。此外，基于体内实验结果，对辣木叶中发现的尼亚齐辛-A、尼亚齐明-A和尼亚齐明-B进行了分子对接研究。结果表明，辣木提取物可治疗舌组织的氧化损伤，尤其是尼亚齐明-A和尼亚齐明-B对髓过氧化物酶（MPO）和乳酸脱氢酶（LDH）表现出高结合亲和力。进一步的研究可能表明，将辣木叶提取物与VPA联合使用可预防对舌组织的潜在负面影响。
