Zhou Yanyan, Huang Haiyan, Yuan Qi, Ren Jingyuan, Wu Jiashen, Zhao Xilin, Lin Yuchun, Lin Zhongning, Xu Ling
State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Public Health, Xiamen University, Xiamen, China.
State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Public Health, Xiamen University, Xiamen, China.
Biomater Adv. 2025 Apr;169:214143. doi: 10.1016/j.bioadv.2024.214143. Epub 2024 Dec 6.
Diabetes mellitus ranks as the eighth most prevalent cause of mortality and disability worldwide. It is a major challenge for clinics to treat diabetic-infected wounds. The hydrogel (referred to as NanoAg@QAC), which combines the advantages of nanosilver (NanoAg) and quaternary ammonium chitosan (QAC), possesses the characteristics of an ideal wound dressing, including proper mechanical properties, antimicrobial activity, anti-biofilm properties, and cytocompatibility. The NanoAg@QAC hydrogel proved to be efficacious in treating infections caused by S. aureus and P. aeruginosa in vivo, thereby promoting wound closure during the initial phase of healing. The application of the NanoAg@QAC hydrogel efficiently suppressed M1-type macrophage marker iNOS expression and simultaneously enhanced the M2-type macrophage marker CD206, which promoted the M1 to M2 transition. The hydrogel significantly reduced the pro-inflammatory cytokine interleukin-1β (IL-1β) and increased the levels of vascular endothelial growth factor A (VEGFA), which alleviated the inflammatory response of the wound and promoted neovascularization. Furthermore, the NanoAg@QAC hydrogel enhanced tissue regeneration and collagen deposition. Thisw study demonstrates that the NanoAg@QAC hydrogel exhibits significant potential for application in the treatment of diabetic-infected wounds.
糖尿病是全球第八大常见的死亡和致残原因。治疗糖尿病感染伤口是临床上的一项重大挑战。结合了纳米银（NanoAg）和季铵化壳聚糖（QAC）优点的水凝胶（称为NanoAg@QAC）具有理想伤口敷料的特性，包括适当的机械性能、抗菌活性、抗生物膜性能和细胞相容性。NanoAg@QAC水凝胶在体内被证明对治疗由金黄色葡萄球菌和铜绿假单胞菌引起的感染有效，从而在愈合初期促进伤口闭合。NanoAg@QAC水凝胶的应用有效抑制了M1型巨噬细胞标志物iNOS的表达，同时增强了M2型巨噬细胞标志物CD206，促进了M1向M2的转变。该水凝胶显著降低了促炎细胞因子白细胞介素-1β（IL-1β）的水平，并提高了血管内皮生长因子A（VEGFA）的水平，减轻了伤口的炎症反应并促进了新血管形成。此外，NanoAg@QAC水凝胶增强了组织再生和胶原蛋白沉积。这项研究表明，NanoAg@QAC水凝胶在治疗糖尿病感染伤口方面具有显著的应用潜力。
