Li Xiaojing, Yang Rong, Bai Zhijun, Gan Pingsheng, Zeng Tao, Liu Miao, Yuan Jun
Guangzhou Center for Disease Control and Prevention, Guanzhou 510440, Guangdong, China.
Guangzhou Center for Disease Control and Prevention, Guanzhou 510440, Guangdong, China.
Food Chem. 2025 Mar 1;467:142361. doi: 10.1016/j.foodchem.2024.142361. Epub 2024 Dec 4.
A sensitive, straightforward, and environmentally sustainable method was developed and validated for the quantification of 13 PBDEs in chicken eggs using gas chromatography coupled with triple quadrupole mass spectrometry (GC-MS/MS). The QuEChERS approach, combined with dispersive liquid-liquid microextraction (DLLME), was applied for sample preparation. Egg samples were extracted with acetonitrile and purified using Z-Sep + adsorbent. Tetrachloroethane was selected as the extraction solvent for the DLLME step. All PBDEs exhibited strong linearity, with R > 0.999 within the range of 0.20-10.0 ng/mL, and the limits of quantification (LOQ) were determined to be 10.0 pg/g. Apparent recoveries of PBDEs ranged from 85.2 % to 105.7 %. A total of 119 chicken eggs were analyzed using the developed method. BDE-47 and BDE-99 showed relatively high detection rates of 38.6 % and 24.4 %, respectively. The hazard quotient (HQ) values for BDE-47, BDE-99, and BDE-153 congeners remained well below 1.0, indicating no significant health risk from egg consumption.
开发并验证了一种灵敏、直接且环境可持续的方法，用于使用气相色谱-三重四极杆质谱联用仪（GC-MS/MS）定量鸡蛋中的13种多溴二苯醚。采用QuEChERS方法结合分散液液微萃取（DLLME）进行样品制备。鸡蛋样品用乙腈萃取，并用Z-Sep +吸附剂净化。选择四氯乙烷作为DLLME步骤的萃取溶剂。所有多溴二苯醚均表现出很强的线性，在0.20-10.0 ng/mL范围内R>0.999，定量限（LOQ）确定为10.0 pg/g。多溴二苯醚的表观回收率在85.2%至105.7%之间。使用所开发的方法对总共119个鸡蛋进行了分析。BDE-47和BDE-99的检出率相对较高，分别为38.6%和24.4%。BDE-47、BDE-99和BDE-153同系物的危害商（HQ）值均远低于1.0，表明食用鸡蛋不存在显著健康风险。
