Wang Jiali, Guan Jiaxin, Jia Fengzhen, Tian Zitong, Song Lili, Xie Lei, Han Pengde, Lin He, Qiao Haixia, Zhang Xuejiao, Huang Yong
College of Lab Medicine, Life Science Research Centre, Hebei North University, Zhangjiakou 075000, China.
School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, China.
Int J Biol Macromol. 2025 Jan;287:138608. doi: 10.1016/j.ijbiomac.2024.138608. Epub 2024 Dec 9.
Failure of orthopedic implants due to localized bacterial infections, inflammation and insufficient blood supply is always problematic. In this study, strontium-doped titanium dioxide nanotubes (STN) were firstly prepared on titanium surface, and then lactoferrin (LF) was loaded into strontium-doped nanotubes (STN) by the phase transition method, eventually the LF/TCEP-STN composite coating was successfully prepared. With the innate antimicrobial properties of LF, LF/TCEP-STN was effected against E. coli and S. aureus. Cellular assays showed that RAW264.7 (immune), HUVEC (angiogenic) and MC3T3-E1 (osteogenic) exhibited good adhesion and proliferative activity on the surface of LF/TCEP-STN. At the molecular level, LF/TCEP-STN modulated RAW264.7 polarization toward M2-type while promoting MC3T3-E1 differentiation toward osteogenesis. Meanwhile LF/TCEP-STN coating effectively promoted angiogenesis. The results of the bone defect model with or without infection demonstrated that the LF/TCEP-STN material had good anti-inflammatory, antibacterial, and vascularization-promoting osteogenesis. In addition, LF/TCEP-STN offered excellent blood compatibility and biosafety. As a multifunctional coating on implant surfaces, the study's results highlighted the viability of LF/TCEP-STN and offered fresh concepts for the clinical design of next-generation artificial bone implants with antibacterial, anti-inflammatory, and osteogenic properties.
由于局部细菌感染、炎症和血液供应不足导致的骨科植入物失效一直是个问题。在本研究中，首先在钛表面制备了掺锶二氧化钛纳米管（STN），然后通过相变法将乳铁蛋白（LF）负载到掺锶纳米管（STN）中，最终成功制备了LF/TCEP-STN复合涂层。凭借LF固有的抗菌特性，LF/TCEP-STN对大肠杆菌和金黄色葡萄球菌有抑制作用。细胞实验表明，RAW264.7（免疫细胞）、HUVEC（血管生成细胞）和MC3T3-E1（成骨细胞）在LF/TCEP-STN表面表现出良好的粘附和增殖活性。在分子水平上，LF/TCEP-STN调节RAW264.7向M2型极化，同时促进MC3T3-E1向成骨分化。同时，LF/TCEP-STN涂层有效促进血管生成。有或无感染的骨缺损模型结果表明，LF/TCEP-STN材料具有良好的抗炎、抗菌和促进血管生成的成骨作用。此外，LF/TCEP-STN具有优异的血液相容性和生物安全性。作为植入物表面的多功能涂层，该研究结果突出了LF/TCEP-STN的可行性，并为具有抗菌、抗炎和成骨特性的下一代人工骨植入物的临床设计提供了新的概念。
