Jia Fengzhen, Guan Jiaxin, Wang Jiali, Li Meiyu, Zhang Yasi, Xie Lei, Han Pengde, Lin He, Huang Xiao, Lan Jinping, Huang Yong
College of Lab Medicine, Life Science Research Centre, Hebei North University, Zhangjiakou 075000, China.
School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114335. doi: 10.1016/j.colsurfb.2024.114335. Epub 2024 Oct 23.
Inflammation and bacterial infection are important causes of implant failure, and the development of multifunctional titanium surfaces to address these issues is an effective means of treating infected bone defects. In this study, polyphenols (EGCG) and Zn were first loaded onto the titanium surface to construct an EGCG/Zn polyphenol metal network coating. Then melatonin (MT) was loaded into the EGCG/Zn network structure to prepare the EGCG/Zn/MT composite coating. The results proved that the EGCG/Zn/MT coating had good mechanical properties, hydrophilicity, corrosion resistance and bioactivity. In vitro, the inhibition rates of EGCG/Zn/MT against E. coli and S. aureus were about 97 % and 81 %, respectively. In vitro experiments revealed that EGCG/Zn/MT could regulate the polarization of macrophages (RAW264.7) to M2 type, could induce vascularization of human umbilical vein endothelial cells (HUVEC), and could promote the differentiation of pro-osteoblasts (MC3T3-E1) to osteogenesis. Meanwhile, EGCG/Zn/MT achieved effective ROS scavenging within HUVEC and MC3T3-E1. In vivo experiments demonstrated that the EGCG/Zn/MT coatings possessed favorable biosafety, anti-inflammatory, antimicrobial, and bone repair capabilities. This study provides a simple and versatile strategy for designing multifunctional surfaces with both antimicrobial, anti-inflammatory, antioxidant, angiogenic and osteogenic properties.
炎症和细菌感染是植入物失败的重要原因，开发多功能钛表面以解决这些问题是治疗感染性骨缺损的有效手段。在本研究中，首先将多酚（表没食子儿没食子酸酯，EGCG）和锌负载到钛表面，构建EGCG/锌多酚金属网络涂层。然后将褪黑素（MT）负载到EGCG/锌网络结构中，制备EGCG/锌/MT复合涂层。结果证明，EGCG/锌/MT涂层具有良好的力学性能、亲水性、耐腐蚀性和生物活性。在体外，EGCG/锌/MT对大肠杆菌和金黄色葡萄球菌的抑制率分别约为97%和81%。体外实验表明，EGCG/锌/MT可将巨噬细胞（RAW264.7）的极化调节为M2型，可诱导人脐静脉内皮细胞（HUVEC）血管生成，并可促进前成骨细胞（MC3T3-E1）向成骨分化。同时，EGCG/锌/MT在HUVEC和MC3T3-E1内实现了有效的活性氧清除。体内实验表明，EGCG/锌/MT涂层具有良好的生物安全性、抗炎、抗菌和骨修复能力。本研究为设计具有抗菌、抗炎、抗氧化、血管生成和成骨特性的多功能表面提供了一种简单通用的策略。
