Brandoli C, Mortada A, Todeschini C, Siniscalco C, Sgarbi E
BIOGEST-SITEIA, Università Di Modena e Reggio Emilia, Via Amendola 2, 42124, Reggio Emilia, Italy.
Department of Chemical and Geological Sciences, Università Di Modena e Reggio Emilia, Via Amendola, 2, 42122, Reggio Emilia, Italy.
Protoplasma. 2025 May;262(3):545-561. doi: 10.1007/s00709-024-02015-z. Epub 2024 Dec 11.
In this work, we propose a possible correlation between carbohydrate content in hazelnut pollen (wild type) and viability/germinability, also in a perspective of adaptation to climate variability. Samples from four different cultivation fields in Italy showed values of pollen viability characterized by high levels, ranging between 77.3 and 98.4% and a unique trend during the flowering period for each accession. When subjected to dehydration in controlled environment, pollen reduced the levels of viability to almost zero but recovered the initial values when rehydrated. The presence of anomalous pollen was found to be not significant, always below 4% in all accessions. The analysis on starch content gave negative results both when it was determined biochemically and detected by histological staining. Sucrose content resulted always higher than glucose and fructose in all the accessions analyzed. Its concentration throughout the dispersal phases reflected the trend of both pollen viability and germinability. These data seem to suggest a direct involvement of sucrose in the protection of plasma membranes from dehydration and the maintenance of pollen viability and germinability. This study demonstrates the sensitivity of hazelnut pollen to climatic fluctuations, particularly to air dry condition, stressing a significant role of sucrose in maintaing viablity and germinabilty during all dispersal period.
在这项工作中，我们提出了榛树花粉（野生型）中碳水化合物含量与活力/发芽能力之间可能存在的关联，也是从适应气候变化的角度出发。来自意大利四个不同种植地的样本显示出花粉活力值处于较高水平，范围在77.3%至98.4%之间，并且每个种质在开花期都有独特的趋势。当在受控环境中进行脱水处理时，花粉活力水平降至几乎为零，但重新水化后又恢复到初始值。发现异常花粉的存在并不显著，在所有种质中均始终低于4%。对淀粉含量的分析，无论是通过生化测定还是组织学染色检测，都得出了阴性结果。在所有分析的种质中，蔗糖含量始终高于葡萄糖和果糖。其在整个传播阶段的浓度反映了花粉活力和发芽能力的趋势。这些数据似乎表明蔗糖直接参与了保护质膜免受脱水影响以及维持花粉活力和发芽能力。本研究证明了榛树花粉对气候波动，特别是对空气干燥条件的敏感性，强调了蔗糖在整个传播期间维持活力和发芽能力方面的重要作用。
