Yang Ruixin, Qi Yao, Zhang Xiaoyan, Gao Hengjun, Yu Yingyan
Department of General Surgery of Ruijin Hospital, Shanghai Institute of Digestive Surgery, and Shanghai Key Laboratory for Gastric Neoplasms, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
National Engineering Center for Biochip at Shanghai, Shanghai 200120, China.
Chin Med J (Engl). 2024 Dec 20;137(24):3050-3060. doi: 10.1097/CM9.0000000000003414. Epub 2024 Dec 12.
In multiple areas such as science, technology, and economic activities, it is necessary to unify the management of repetitive tasks or concepts by standardization to obtain the best order and high efficiency. Organoids, as living tissue models, have rapidly developed in the past decade. Organoids can be used repetitively for in vitro culture, cryopreservation, and recovery for further utilization. Because organoids can recapitulate the parental tissues' morphological phenotypes, cell functions, biological behaviors, and genomic profiles, they are known as renewable "living biobanks". Organoids cover two mainstream fields: Adult stem cell-derived organoids (also known as patient-derived organoids) and induced pluripotent stem cell-derived and/or embryonic stem cell-derived organoids. Given the increasing importance of organoids in the development of new drugs, standardized operation, and management in all steps of organoid construction is an important guarantee to ensure the high quality of products. In this review, we systematically introduce the standardization of organoid construction operation procedures, the standardization of laboratory construction, and available standardization documents related to organoid culture that have been published so far. We also proposed the challenges and prospects in this field.
在科学、技术和经济活动等多个领域，有必要通过标准化对重复性任务或概念进行统一管理，以获得最佳秩序和高效率。类器官作为活组织模型，在过去十年中迅速发展。类器官可重复用于体外培养、冷冻保存和复苏以供进一步利用。由于类器官能够重现亲代组织的形态表型、细胞功能、生物学行为和基因组特征，它们被称为可再生的“活生物样本库”。类器官涵盖两个主流领域：成体干细胞衍生的类器官（也称为患者来源的类器官）以及诱导多能干细胞衍生的和/或胚胎干细胞衍生的类器官。鉴于类器官在新药开发中的重要性日益增加，类器官构建各步骤的标准化操作和管理是确保产品高质量的重要保证。在这篇综述中，我们系统地介绍了类器官构建操作程序的标准化、实验室建设的标准化以及迄今为止已发表的与类器官培养相关的可用标准化文件。我们还提出了该领域的挑战和前景。
