Aliyev Huseyn, Bilgili Sibel, Toktay Erdem, Nuriyeva Nubar, Bayir Yasin
Department of Pharmacuitical chemistry, Azerbaijan Medical University Faculty of Pharmacy, Baku, Azerbaijan.
Department of Mathematics Education, Ataturk University Faculty of Kazım Karabekir Education, Erzurum, Türkiye.
Eurasian J Med. 2024 Sep 13;57(1):1-7. doi: 10.5152/eurasianjmed.2024.23214.
The aim of this study is to examine the protective effect of oxyresveratrol (OXY) against isoproterenol-induced myocardial infarction in rats, through routine biochemical parameters and oxidative stress parameters that show heart damage. Oxyresveratrol was administered by oral gavage at doses of 10 and 20 mg/kg, respectively, once a day for 5 days. On the fourth and fifth days, 180 mg/kg isoproterenol was administered intraperitoneally to the OXY treatment group and control groups. Twenty-four hours after the last isoproterenol application, blood and heart tissue were taken under anesthesia and transferred to -80 degrees and formalin for biochemical and histopathological studies. CK-MB and TnI levels were measured in serum obtained from blood. In the heart tissue, antioxidant parameters, superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) levels, and histopathological and stereological evaluations were performed. Oxyresveratrol has antioxidant and anti-inflammatory activity depending on the dose. Oxyresveratrol showed potent protective effect against isoproterenol-induced myocardial infarction. It has been proven that at all doses of oxyresveratrol, statistically, isoproterenol decreased the MDA level, which was one of the oxidative stress markers, compared to the control group, and increased SOD activity and GSH levels. Similar to the biochemically determined parameters, oxyresveratrol treatment was also found to have a protective effect at the cell level, histopathologically and stereologically. All results show that oxyresveratrol has strong antioxidant and anti-inflammatory activity, especially at a dose of 20 mg/kg, it significantly reduces myocardial damage and this agent has a cardioprotective effect.
本研究旨在通过显示心脏损伤的常规生化参数和氧化应激参数，研究氧化白藜芦醇（OXY）对异丙肾上腺素诱导的大鼠心肌梗死的保护作用。氧化白藜芦醇分别以10和20mg/kg的剂量通过口服灌胃给药，每天一次，共5天。在第四和第五天，向OXY治疗组和对照组腹腔注射180mg/kg异丙肾上腺素。在最后一次应用异丙肾上腺素24小时后，在麻醉下采集血液和心脏组织，并转移至-80℃和福尔马林中进行生化和组织病理学研究。测定血液中血清的肌酸激酶同工酶（CK-MB）和肌钙蛋白I（TnI）水平。在心脏组织中，进行抗氧化参数、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和丙二醛（MDA）水平的测定，以及组织病理学和体视学评估。氧化白藜芦醇具有剂量依赖性的抗氧化和抗炎活性。氧化白藜芦醇对异丙肾上腺素诱导的心肌梗死显示出强大的保护作用。已证明，在所有氧化白藜芦醇剂量下，与对照组相比，异丙肾上腺素在统计学上降低了作为氧化应激标志物之一的MDA水平，并增加了SOD活性和GSH水平。与生化测定参数相似，氧化白藜芦醇治疗在组织病理学和体视学的细胞水平上也具有保护作用。所有结果表明，氧化白藜芦醇具有强大的抗氧化和抗炎活性，尤其是在20mg/kg的剂量下，它能显著减少心肌损伤，且该药物具有心脏保护作用。
