Huang Xiaolong, Li Haibo, Zhang Lan, Zhang Xu, Cheng Shaochuan, Yan Yuying, Yang Wei, Meng Bingshun, Wang Zuobo, Zhao Juanjuan, Ran Jingcheng
Guizhou Academy of Forestry Guiyang China.
Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China Guizhou Academy of Forestry Guiyang China.
Ecol Evol. 2024 Dec 10;14(12):e70690. doi: 10.1002/ece3.70690. eCollection 2024 Dec.
Maintaining a healthy status is crucial for the successful captive breeding of critically endangered , it is conducive to ex situ conservation of this species and rejuvenation of its population. However, changes in the feeding environment and food can affect the composition and function of the gut microbiota in , ultimately impacting its health and adaptation. Herein, 16S rRNA gene sequencing was employed to determine the gut microbiota composition and functional variations between wild and captive populations. The results showed that the captive group had higher alpha diversity than the wild group, and significant differences were observed in their beta diversity. Captive and wild showed similar microbiota at the phylum level, which mainly comprised Firmicutes, Bacteroidota, and Spirochaetota, but captivity reduced the Firmicutes/Bacteroides ratio. Differential abundance analysis revealed that the relative abundance of microbiota related to cellulose degradation, such as Prevotellaceae_UCG_001, Christensenellaceae_R_7_group, , and , differed significantly between the two groups. Furthermore, the potential pathogens and were significantly abundant in wild and captive groups, respectively. Functional predictions demonstrated that the most significant functional pathways at the second level between captive and wild monkeys were carbohydrate, amino acid, and lipid metabolisms. The captive monkeys exhibited higher digestive capacity and endocrine regulation as well as a higher risk of infectious diseases than wild monkeys. These findings can serve as a valuable theoretical basis for promoting the healthy breeding of and as a guide for future evaluation of the health of wild and captive monkeys.
保持健康状态对于极度濒危物种的成功圈养繁殖至关重要，这有利于该物种的迁地保护及其种群的复兴。然而，饲养环境和食物的变化会影响该物种肠道微生物群的组成和功能，最终影响其健康和适应性。在此，采用16S rRNA基因测序来确定野生和圈养该物种种群之间的肠道微生物群组成和功能变化。结果表明，圈养组的α多样性高于野生组，并且在β多样性方面观察到显著差异。圈养和野生该物种在门水平上显示出相似的微生物群，主要包括厚壁菌门、拟杆菌门和螺旋体门，但圈养降低了厚壁菌门/拟杆菌门的比例。差异丰度分析显示，两组之间与纤维素降解相关的微生物群的相对丰度存在显著差异，如普雷沃氏菌科_UCG_001、克里斯滕森菌科_R_7_group等。此外，潜在病原体在野生组和圈养组中分别显著丰富。功能预测表明，圈养和野生猴子在二级水平上最显著的功能途径是碳水化合物、氨基酸和脂质代谢。与野生猴子相比，圈养猴子表现出更高的消化能力和内分泌调节能力以及更高的传染病风险。这些发现可为促进该物种的健康繁殖提供有价值的理论基础，并为未来评估野生和圈养猴子的健康状况提供指导。
