Mo Fengxiang, Li Yuhui, Liu Zheng, Zheng Jingjin, Huang Zhonghao
Key Laboratory of Ecology and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, China.
Guangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin, China.
Front Microbiol. 2023 May 12;14:1166688. doi: 10.3389/fmicb.2023.1166688. eCollection 2023.
Gut microbiota is crucial to primate survival. Data on the gut microbiota of captive and wild animals can provide a physiological and ecological basis for the conservation of rare and endangered species. To study the effect of captivity on the gut microbiota, we examine the difference in the gut microbiota composition between captive and wild Francois' langurs (), using 16S rRNA sequencing technology. The results showed that the composition of the gut microbiota of captive and wild langurs was characterized by Firmicutes (51.93 ± 10.07% vs. 76.15 ± 8.37%) and Bacteroidetes (32.43 ± 10.00% vs. 4.82 ± 1.41%) at the phylum level and was characterized by Oscillospiraceae (15.80 ± 5.19% vs. 30.21 ± 4.87%) at the family level. The alpha diversity of gut microbiota in captive langurs was higher than those in wild, such as the Shannon index (4.45 ± 0.33 vs. 3.98 ± 0.19, < 0.001) and Simpson index (35.11 ± 15.63 vs. 19.02 ± 4.87, < 0.001). Principal coordinates analysis (PCoA) results showed significant differences in the composition of gut microbiota between captive and wild langurs at both the phylum and family levels (weight UniFrac algorithm, phylum level: = 0.748, = 0.001; family level: = 0.685, = 0.001). The relative abundance of Firmicutes (51.93 ± 10.07%) in captive langurs was lower than that of wild langurs (76.15 ± 8.37%), and the relative abundance of Bacteroidetes (32.43 ± 10.00%) in captive langurs was higher than that of wild (4.82 ± 1.41%). Our study concludes that dietary composition could be a crucial determinant in shaping the gut microbiota of langurs because more fiber-rich foods used by the wild langurs could increase the abundance of Firmicutes, and more simple carbohydrate-rich foods consumed by the captive langurs increase the abundance of Bacteroidetes. We highlight the importance of captivity on the gut microbiota and the need to consider the gut microbiota in animal provision.
肠道微生物群对灵长类动物的生存至关重要。圈养动物和野生动物的肠道微生物群数据可为珍稀濒危物种的保护提供生理和生态基础。为了研究圈养对肠道微生物群的影响，我们使用16S rRNA测序技术，检测了圈养和野生黑叶猴肠道微生物群组成的差异。结果表明，在门水平上，圈养和野生黑叶猴肠道微生物群的组成以厚壁菌门（51.93±10.07%对76.15±8.37%）和拟杆菌门（32.43±10.00%对4.82±1.41%）为特征；在科水平上，以颤螺菌科（15.80±5.19%对30.21±4.87%）为特征。圈养黑叶猴肠道微生物群的α多样性高于野生黑叶猴，如香农指数（4.45±0.33对3.98±0.19，P<0.001）和辛普森指数（35.11±15.63对19.02±4.87，P<0.001）。主坐标分析（PCoA）结果表明，圈养和野生黑叶猴在门和科水平上的肠道微生物群组成存在显著差异（加权UniFrac算法，门水平：R=0.748，P=0.001；科水平：R=0.685，P=0.001）。圈养黑叶猴中厚壁菌门的相对丰度（51.93±10.07%）低于野生黑叶猴（76.15±8.37%），圈养黑叶猴中拟杆菌门的相对丰度（32.43±10.00%）高于野生黑叶猴（4.82±1.41%）。我们的研究得出结论，饮食组成可能是塑造黑叶猴肠道微生物群的关键决定因素，因为野生黑叶猴食用的富含纤维的食物更多，这可能会增加厚壁菌门的丰度，而圈养黑叶猴食用的富含简单碳水化合物的食物更多，会增加拟杆菌门的丰度。我们强调了圈养对肠道微生物群的重要性，以及在动物饲养中考虑肠道微生物群的必要性。
