El Amri Mohamed, Pandit Abhay, Schlosser Gerhard
School of Biological and Chemical Sciences, University of Galway, Galway, Ireland.
Research Ireland Center for Medical Devices (CÚRAM), University of Galway, Galway, Ireland.
Elife. 2024 Dec 12;13:e98277. doi: 10.7554/eLife.98277.
Marcks and Marcksl1 are abundant proteins that shuttle between the cytoplasm and membrane to modulate multiple cellular processes, including cytoskeletal dynamics, proliferation, and secretion. Here, we performed loss- and gain-of-function experiments in to reveal the novel roles of these proteins in spinal cord development and regeneration. We show that Marcks and Marcksl1 have partly redundant functions and are required for normal neurite formation and proliferation of neuro-glial progenitors during embryonic spinal cord development and for its regeneration during tadpole stages. Rescue experiments in Marcks and Marcksl1 loss-of-function animals further suggested that some of the functions of Marcks and Marcksl1 in the spinal cord are mediated by phospholipid signaling. Taken together, these findings identify Marcks and Marcksl1 as critical new players in spinal cord development and regeneration and suggest new pathways to be targeted for therapeutic stimulation of spinal cord regeneration in human patients.
Marcks和Marcksl1是丰富的蛋白质，它们在细胞质和细胞膜之间穿梭，调节多种细胞过程，包括细胞骨架动力学、增殖和分泌。在这里，我们在[具体对象未提及]中进行了功能丧失和功能获得实验，以揭示这些蛋白质在脊髓发育和再生中的新作用。我们发现，Marcks和Marcksl1具有部分冗余功能，在胚胎脊髓发育过程中，对于正常神经突形成和神经胶质祖细胞的增殖是必需的，在蝌蚪阶段对于脊髓再生也是必需的。在Marcks和Marcksl1功能丧失动物中进行的拯救实验进一步表明，Marcks和Marcksl1在脊髓中的一些功能是由磷脂信号介导的。综上所述，这些发现确定Marcks和Marcksl1是脊髓发育和再生中的关键新参与者，并提出了针对人类患者脊髓再生治疗刺激的新途径。
