Srinivas Pooja, Peterson S Brook, Gallagher Larry A, Wang Yaxi, Mougous Joseph D
Department of Microbiology, University of Washington, Seattle, WA 98109.
HHMI, University of Washington, Seattle, WA 98109.
Proc Natl Acad Sci U S A. 2024 Dec 17;121(51):e2419369121. doi: 10.1073/pnas.2419369121. Epub 2024 Dec 12.
Patescibacteria, also known as the Candidate Phyla Radiation, are a diverse clade of largely uncultivated, small bacteria that comprise a significant proportion of all bacterial diversity. The few members that have been cultivated exhibit a fascinating life cycle in which they grow as obligate epibionts on the surface of host bacteria. In this Perspective, we make the case that the study of these unique, divergent, and poorly characterized organisms represents an exciting frontier in microbiology. This burgeoning field has already achieved several critical breakthroughs, including metagenomic sequence-based reconstructions of the metabolic and biosynthetic capabilities of diverse Patescibacteria and the development of generalizable strategies for their cultivation and genetic manipulation. We argue these that advances, among others, should pave the way toward a molecular understanding of the complex interactions that undoubtedly underpin the relationship between Patescibacteria and their hosts.
原壁菌门，也被称为候选门辐射类群，是一类多样的、大多未培养的小型细菌，在所有细菌多样性中占很大比例。少数已培养的成员展现出一种迷人的生命周期，它们作为专性外共生菌在宿主细菌表面生长。在这篇观点文章中，我们认为对这些独特、分化且特征不明的生物体的研究代表了微生物学中一个令人兴奋的前沿领域。这个新兴领域已经取得了几个关键突破，包括基于宏基因组序列对多种原壁菌门的代谢和生物合成能力进行重建，以及开发出适用于它们培养和基因操作的通用策略。我们认为，这些进展以及其他方面的进展，应该为从分子层面理解无疑支撑着原壁菌门与其宿主之间关系的复杂相互作用铺平道路。
