Stachniuk Anna, Kozub Anna, Czeczko Renata, Montowska Magdalena, Fornal Emilia
Department of Bioanalytics, Medical University of Lublin, ul. Jaczewskiego 8b, 20-090, Lublin, Poland.
Department of Chemistry, University of Live Sciences in Lublin, ul. Akademicka 15, 20-950, Lublin, Poland.
J Food Drug Anal. 2022 Jun 15;30(2):223-233. doi: 10.38212/2224-6614.3403.
Ten rabbit-specific tryptic peptide markers and one marker peptide specific to both rabbit and hare were evaluated for mass signal linearity in binary meat mixtures using liquid chromatography-quadrupole time-of-flight-mass spectrometry. Seven meat mixtures containing chicken and varying percentages of rabbit (1%, 5%, 10%, 30%, 60%, 90%, and 100%) were analyzed. Additionally, the signal linearity of twelve peptide markers for chicken meat was examined. The best candidate peptides for the quantification of meat content were selected. Five of eleven peptides for rabbit meat and five of twelve peptides for chicken meat showed good linearity (R > 0.97). The limits of detection and limits of quantification for these markers were in the range of 0.43-1.91% [w/w] and 1.44-6.38% [w/w], respectively. The method allowed determination of the percentage content of rabbit and chicken meat in two- and three-component meat mixtures with good accuracy. The preliminary quantification data provide a starting point for developing label-free and absolute quantification methods for rabbit and chicken meat using multiple reaction monitoring of peptide markers.
使用液相色谱 - 四极杆飞行时间质谱法，对10种兔特异性胰蛋白酶肽标记物和1种兔和野兔共有的标记肽在二元肉类混合物中的质量信号线性进行了评估。分析了7种含有鸡肉和不同百分比兔肉（1%、5%、10%、30%、60%、90%和100%）的肉类混合物。此外，还检测了12种鸡肉肽标记物的信号线性。选择了用于定量肉类含量的最佳候选肽。11种兔肉肽中的5种和12种鸡肉肽中的5种显示出良好的线性（R>0.97）。这些标记物的检测限和定量限分别在0.43 - 1.91%[w/w]和1.44 - 6.38%[w/w]范围内。该方法能够准确测定二元和三元肉类混合物中兔肉和鸡肉的百分比含量。初步定量数据为利用肽标记物的多反应监测开发兔肉和鸡肉的无标记绝对定量方法提供了一个起点。
