College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province 266003, People's Republic of China.
College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province 266003, People's Republic of China.
Food Chem. 2019 Mar 15;276:358-365. doi: 10.1016/j.foodchem.2018.10.014. Epub 2018 Oct 3.
The availability of analytical methods for quantification of allergens is crucial for the correct assessment and labeling of products in order to protect allergic consumers. For this purpose, a simple, sensitive and accurate technique was developed based on liquid chromatography-tandem mass spectrometry and multiple reaction monitoring mass spectrometry (LC-MRM-MS/MS). The proposed method uses a simple purification with heat and a completely optimized tryptic digestion. This method has been validated according to the requirements defined by ICH (Q2 [R1]), having a linear range from 0.10 to 1179.36 nM with r > 0.999. The parvalbumin beta in flounder (Paralichthys olivaceus) has been quantified at a low level down to 0.10 µg/g with satisfactory precision (RSD < 18.35%) and accuracy (<13.3%). The new approach was successfully applied for the determination of parvalbumin beta in the other food matrices.
过敏原分析方法的可用性对于正确评估和标记产品以保护过敏消费者至关重要。为此，开发了一种基于液相色谱-串联质谱和多重反应监测质谱（LC-MRM-MS/MS）的简单、灵敏和准确的技术。该方法采用简单的热净化和完全优化的胰蛋白酶消化。该方法已根据 ICH（Q2 [R1]）的要求进行验证，线性范围为 0.10 至 1179.36 nM，r > 0.999。已经在低水平下定量了比目鱼（Paralichthys olivaceus）中的副肌球蛋白β，达到 0.10 µg/g，具有令人满意的精密度（RSD < 18.35%）和准确性（<13.3%）。该新方法成功应用于其他食品基质中副肌球蛋白β的测定。
