2019冠状病毒病患者循环微小RNA及其在炎症调节中的作用研究。

Investigation of the circulatory microRNAs and their involvement in regulation of inflammation in patients with COVID-19.

作者信息

Abbasifard Mitra, Ostad Ebrahimi Hamid, Taghipur Khajeh Sharifi Gholamhosein, Bahrehmand Fatemeh, Bagheri-Hosseinabadi Zahra

机构信息

Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran; Department of Internal Medicine, Ali-Ibn Abi-Talib Hospital, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

Department of Paediatrics, Ali-Ibn Abi-Talib Hospital, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

出版信息

Hum Immunol. 2025 Jan;86(1):111208. doi: 10.1016/j.humimm.2024.111208. Epub 2024 Dec 11.

DOI:10.1016/j.humimm.2024.111208

PMID:39667207

Abstract

BACKGROUND

Dysregulated levels of cytokines may lead to cytokine storm, which has been implicated in the immunopathogenesis of coronavirus disease 2019 (COVID-19). Here in the current study, the role of microRNA (miR)-155-5p, miR-146a, and miR-221-3p in the regulation of the immune responses and inflammatory state in patients with COVID-19 was investigated.

METHODS

In this case-control study, peripheral blood samples were obtained from 75 COVID-19 subjects and 100 healthy controls. From the plasma samples, RNA was extracted and cDNA was synthesized, and subsequently the transcript level of miRNAs was measured by Real-time PCR. The plasma levels of interleukin (IL)-4 and interferon (IFN)-γ were determined using ELISA.

RESULTS

miR-155-5p (fold change = 1.87, P = 0.020) and miR-221-3p (fold change = 2.26, P = 0.008), but not miR-146a, was upregulated in the plasma sample of COVID-19 cases compared to controls. The level of IFN-γ (but not IL-4) was significantly higher in the plasma samples of COVID-19 patients compared to control group. The expression level of miR-155-5p (r = 0.35, corrected P = 0.066) and miR-221-3p (r = 0.25, corrected P = 0.066) had positive correlation with the plasma levels of IFN-γ.

CONCLUSIONS

IFN-γ pathway in involved in the pathogenesis of COVID-19 that is regulated through miR-155-5p and miR-221-3p. These miRNAs showed potential utility as biomarkers for predicting the severity of COVID-19.

摘要

背景

细胞因子水平失调可能导致细胞因子风暴，这与2019冠状病毒病（COVID-19）的免疫发病机制有关。在本研究中，研究了微小RNA（miR）-155-5p、miR-146a和miR-221-3p在调节COVID-19患者免疫反应和炎症状态中的作用。

方法

在这项病例对照研究中，从75名COVID-19患者和100名健康对照者中采集外周血样本。从血浆样本中提取RNA并合成cDNA，随后通过实时PCR测量miRNA的转录水平。使用酶联免疫吸附测定法测定血浆中白细胞介素（IL）-4和干扰素（IFN）-γ的水平。

结果

与对照组相比，COVID-19患者血浆样本中miR-155-5p（倍数变化=1.87，P=0.020）和miR-221-3p（倍数变化=2.26，P=0.008）上调，而miR-146a未上调。与对照组相比，COVID-19患者血浆样本中IFN-γ（而非IL-4）水平显著更高。miR-155-5p（r=0.35，校正P=0.066）和miR-221-3p（r=0.25，校正P=0.066）的表达水平与血浆中IFN-γ水平呈正相关。