Hashemi Negin, Tsochatzis Emmanouil, Rasmussen Martin Krøyer, Corredig Milena
Department of Food Science, Aarhus University, Agro Food Park 48, 8200 Aarhus N, Denmark.
Department of Food Science, Aarhus University, Agro Food Park 48, 8200 Aarhus N, Denmark.
Food Chem. 2025 Mar 15;468:142384. doi: 10.1016/j.foodchem.2024.142384. Epub 2024 Dec 5.
In this study, the bioaccessibility and bioavailability of curcumin encapsulated into different lupin protein isolate-based carriers was evaluated by coupling an in vitro gastrointestinal digestion (INFOGEST) with an in vitro co-culture absorption model, Caco-2/HT29-MTX, consisting of both absorptive and mucus producing cells. A targeted ultrahigh-performance quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) method was applied to monitor the fate of curcumin post digestion and absorption, specifically analyzing the apical, cellular, and basolateral fractions. Lupin protein nanoparticles, obtained by desolvation, protected curcumin from degradation better than oil in water (O/W) emulsions stabilized with lupin protein isolate. A recovery of 70 % of initial curcumin was found in the whole digesta of nanoparticles, whereas the emulsion systems displayed ≤35 % recovery. Interestingly, unlike in the case of emulsions, where curcumin was found in the micellar phase, most of the curcumin in the digesta of nanoparticles was recovered in the insoluble phase, highlighting the influence of the matrix structure in ensuring bioaccessibility of bioactive components. Regardless of the treatment, curcumin was not detected in the basolateral compartment, after absorption and transport through the in vitro cell monolayer model. However, a noteworthy proportion of curcumin, 54 % for protein nanoparticles and ≤ 24 % for emulsions, was retrieved within the cell monolayer. Non-targeted metabolomics analysis revealed the presence of a range of curcumin metabolites in the basolateral fraction and showed distinct profiles depending on the type (structure) of the delivery systems. The study highlights the critical need for thorough research into the behavior of bioactive compounds within the gut and emphasizes the necessity for future studies aimed at gaining a deeper understanding of the impact of the food matrix. Such insights are vital for enhancing and optimizing the delivery of bioactive compounds from complex food sources.
在本研究中，通过将体外胃肠道消化（INFOGEST）与由吸收性细胞和黏液分泌细胞组成的体外共培养吸收模型Caco-2/HT29-MTX相结合，评估了包封于不同基于羽扇豆分离蛋白的载体中的姜黄素的生物可及性和生物利用度。采用靶向超高效四极杆飞行时间质谱（UHPLC-QTOF-MS）方法监测姜黄素消化和吸收后的去向，具体分析顶端、细胞内和基底外侧部分。通过去溶剂化获得的羽扇豆蛋白纳米颗粒比用羽扇豆分离蛋白稳定的水包油（O/W）乳液能更好地保护姜黄素不被降解。在纳米颗粒的整个消化物中发现初始姜黄素的回收率为70%，而乳液体系的回收率≤35%。有趣的是，与乳液情况不同，在乳液中姜黄素存在于胶束相中，纳米颗粒消化物中的大部分姜黄素在不溶相中被回收，这突出了基质结构对确保生物活性成分生物可及性的影响。无论采用何种处理方式，在通过体外细胞单层模型吸收和转运后，在基底外侧隔室中均未检测到姜黄素。然而，在细胞单层中回收了相当比例的姜黄素，蛋白纳米颗粒为54%，乳液为≤24%。非靶向代谢组学分析揭示了基底外侧部分存在一系列姜黄素代谢物，并显示出取决于递送系统类型（结构）的不同谱图。该研究强调了对生物活性化合物在肠道内行为进行深入研究的迫切需求，并强调了未来旨在更深入了解食物基质影响的研究的必要性。这些见解对于增强和优化从复杂食物来源递送生物活性化合物至关重要。
