Zhang Xizi, Chen Wei, Wang Chengtao
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing, 100048, PR China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing, 100048, PR China.
Food Microbiol. 2025 Apr;127:104698. doi: 10.1016/j.fm.2024.104698. Epub 2024 Nov 30.
Fungal hyphae self-assemble a variety of cellular macrostates, ranging from suspended mycelium to dense pellets, all inextricably linked to their productivity. In this study, using CRISPR/Cas technology, we constructed a ctnA knockout strain (ΔctnA) and an overexpression strain (A2) so as to investigate the effects of interfering with citrinin biosynthesis on the growth, morphology and pigmentation of M.purpureus. Results indicated that deletion of ctnA in M. purpureus RP2 led to increased mycelium length, delayed conidium formation, and a citrinin content of 22% of the wild-type strain. Conversely, ctnA overexpression in strain A2 resulted in delayed mycelial growth, normal conidium formation, and a citrinin content of 120% compared to the wild-type strain, with minimal effects on pigments content. Notably, the ΔctnA strain formed small, tightly structured pellets (mean diameter 1.2 ± 0.06 mm) and exhibited low citrinin content, promoting pigments production. Our findings suggest a complex interplay between citrinin biosynthesis and morphological development, providing insights for optimizing metabolite production in industrial applications.
真菌菌丝体自组装形成各种细胞宏观状态，从悬浮菌丝体到致密菌球，所有这些都与它们的生产力有着千丝万缕的联系。在本研究中，我们使用CRISPR/Cas技术构建了一个ctnA基因敲除菌株（ΔctnA）和一个过表达菌株（A2），以研究干扰桔霉素生物合成对紫红曲霉生长、形态和色素沉着的影响。结果表明，紫红曲霉RP2中ctnA基因的缺失导致菌丝体长度增加、分生孢子形成延迟，桔霉素含量为野生型菌株的22%。相反，菌株A2中ctnA基因的过表达导致菌丝体生长延迟、分生孢子形成正常，桔霉素含量是野生型菌株的120%，对色素含量影响最小。值得注意的是，ΔctnA菌株形成了小的、结构紧密的菌球（平均直径1.2±0.06毫米），并且桔霉素含量低，促进了色素的产生。我们的研究结果表明桔霉素生物合成与形态发育之间存在复杂的相互作用，为优化工业应用中的代谢产物生产提供了见解。
