He Ji, Zhang Yanjun, Dong Jie, Zhang Wei, Zhu Faming
Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Jianye Road 789, Hangzhou, Zhejiang 310052, China.
Zhejiang Provincial Centers for Disease Control and Prevention, Bingsheng Road 3399, Hangzhou, Zhejiang 310052, China.
Can J Infect Dis Med Microbiol. 2024 Dec 5;2024:4366502. doi: 10.1155/cjid/4366502. eCollection 2024.
To detect and analyze coronavirus SARS-CoV-2 antibody levels in convalescent plasma from donors who have recovered from COVID-19. Plasma samples from 88 donors aged 20-54 years who were diagnosed with COVID-19 and who were eligible to donate from Zhejiang Province, China, were collected as the experimental group, and 56 samples from healthy blood donors were used as controls. Anti-SARS-CoV-2 antibodies, including Ab and IgM, were detected via chemiluminescent immunoassay, and neutralizing antibodies were measured via the microneutralization method. The positive rates of total and IgM antibodies against SARS-CoV-2 were 97.7% (86/88) and 52.3% (46/88), respectively, in the plasma samples of 88 patients who recovered from COVID-19. After 160 and 320 dilutions of the total antibody-positive samples, the positive rates were 61.6% (53/86) and 39.5% (34/86), respectively. The titer of neutralizing antibodies was 16-256 in 53 SARS-CoV-2-positive samples after 160-fold dilution of total antibodies. The titer of neutralizing antibody was 48-256 in 34 samples that were still positive after 320-fold dilution of total antibody. Among the 88 samples, 86 had titers > 4, and 10 had high titers > 80. In 2 patients with neutralizing antibody titers < 4, SARS-CoV-2 total antibody and IgM antibodies were negative. The correlation coefficient between total antibody strength and neutralizing antibody titer was 0.5198 (high correlation). The total antibody and IgM antibodies of 56 healthy blood donors were negative. There are individual differences in plasma antibody titers among convalescent patients. Antibody detection is helpful for screening out plasma with high antibody titers for the treatment of patients with severe disease.
检测和分析新冠康复者恢复期血浆中新型冠状病毒SARS-CoV-2抗体水平。收集来自中国浙江省88名年龄在20 - 54岁、被诊断为新冠且符合献血条件的献血者的血浆样本作为实验组，56份健康献血者的样本作为对照。通过化学发光免疫分析法检测抗SARS-CoV-2抗体，包括抗体和IgM，并用微量中和法检测中和抗体。在88例新冠康复患者的血浆样本中，抗SARS-CoV-2总抗体和IgM抗体的阳性率分别为97.7%（86/88）和52.3%（46/88）。总抗体阳性样本经160倍和320倍稀释后，阳性率分别为61.6%（53/86）和39.5%（34/86）。53份总抗体160倍稀释后的SARS-CoV-2阳性样本中，中和抗体滴度为16 - 256。34份总抗体320倍稀释后仍为阳性的样本中，中和抗体滴度为48 - 256。88份样本中，86份滴度>4，10份高滴度>80。2例中和抗体滴度<4的患者，SARS-CoV-2总抗体和IgM抗体均为阴性。总抗体强度与中和抗体滴度的相关系数为0.5198（高度相关）。56名健康献血者的总抗体和IgM抗体均为阴性。康复患者血浆抗体滴度存在个体差异。抗体检测有助于筛选出高抗体滴度血浆用于重症患者治疗。
