Previtalli-Silva Henrique, Hardoim Daiana de Jesus, Banaggia Raphael de Lucena, Moragas-Tellis Carla Junqueira, Chagas Maria do Socorro Dos Santos, Behrens Maria Dutra, Dias-Silva Thiago de Souza, Calabrese Kátia da Silva, Cardoso Flávia de Oliveira
Laboratory of Protozoology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Av. Brasil, 4.365 - Manguinhos, CEP: 21040-360, Rio de Janeiro, RJ, Brazil.
Postgraduate Program in Tropical Medicine, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Av. Brasil, 4.365 - Manguinhos, CEP: 21040-360, Rio de Janeiro, RJ, Brazil.
Heliyon. 2024 Nov 19;10(23):e40510. doi: 10.1016/j.heliyon.2024.e40510. eCollection 2024 Dec 15.
Mart. is a tropical palm tree native to the Amazon region. Its fruit, commonly known as açaí, has gained widespread recognition for its therapeutic potential, driving the expansion of pharmacological studies to validate its traditional uses. Leveraging açaí seeds in research not only mitigates environmental impacts but also enables the identification of bioactive compounds with potential pharmacological applications, including drug development. Thus, the present work aims to investigate the antioxidant and anti-inflammatory activities of the hydroalcoholic extract and the ethyl acetate, hexane, dichloromethane and aqueous fractions of açaí seeds in vitro. The extract/fractions from açaí contained a significant amount of flavonoids, such as catechins and procyanidins, according to LC-MS/MS. These bioproducts showed significant antioxidant activity, with emphasis on the ethyl acetate fraction (FRAP: 4516.00 ± 58.07 Eq Trolox/g compound; DPPH: IC 3.93 ± 0.26 μg/. mL; ABTS : IC 34.65 ± 0.35 μg/mL). In addition, the compounds exhibited an anti-inflammatory action on LPS-stimulated peritoneal macrophages, with the dichloromethane fraction showing the more comprehensive inhibitory effects on NO, IL-12 and IFN-γ production, especially in the concentration of 500 μg/mL. seeds extract/fractions had no cytotoxic effect on peritoneal macrophages (IC > 500 μg/mL). These findings suggest that seed-derived bioproducts hold significant promise as safe and effective agents for the development of novel antioxidant and anti-inflammatory therapies targeting a variety of inflammatory pathologies.
玛氏棕是一种原产于亚马逊地区的热带棕榈树。其果实，通常被称为阿萨伊果，因其治疗潜力而获得广泛认可，推动了药理学研究的扩展以验证其传统用途。在研究中利用阿萨伊种子不仅可以减轻环境影响，还能够鉴定出具有潜在药理学应用（包括药物开发）的生物活性化合物。因此，本研究旨在体外研究阿萨伊种子的水醇提取物以及乙酸乙酯、己烷、二氯甲烷和水相馏分的抗氧化和抗炎活性。根据液相色谱 - 质谱/质谱分析，阿萨伊提取物/馏分含有大量黄酮类化合物，如儿茶素和原花青素。这些生物制品显示出显著的抗氧化活性，其中乙酸乙酯馏分尤为突出（铁离子还原抗氧化能力法：4516.00±58.07当量 Trolox/克化合物；二苯基苦味酰基自由基法：半数抑制浓度为3.93±0.26微克/毫升；2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐法：半数抑制浓度为34.65±0.35微克/毫升）。此外，这些化合物对脂多糖刺激的腹腔巨噬细胞具有抗炎作用，二氯甲烷馏分对一氧化氮、白细胞介素-12和干扰素-γ的产生表现出更全面的抑制作用，尤其是在浓度为500微克/毫升时。种子提取物/馏分对腹腔巨噬细胞没有细胞毒性作用（半数抑制浓度>500微克/毫升）。这些发现表明，源自阿萨伊种子的生物制品作为开发针对多种炎症性疾病的新型抗氧化和抗炎疗法的安全有效试剂具有巨大潜力。
