Wang Ruiqi, Tian Yun
Department of Traditional Chinese Medicine, Shaanxi Provincial Hospital of Traditional Chinese Medicine, Xi'an, 710003, China.
Department of Nephrology, Shaanxi Provincial Hospital of Traditional Chinese Medicine, No. 4, Xihuamen, Xi'an, Shaanxi, 710003, China.
Open Med (Wars). 2024 Dec 9;19(1):20241042. doi: 10.1515/med-2024-1042. eCollection 2024.
To investigate the correlation between blood stasis syndrome and arteriopathy in immunoglobulin A nephropathy (IgAN). Wall thickness/outer vessel diameter, intimal thickness/outer vessel diameter, and medial thickness/outer vessel diameter were measured using ImageJ software. Vascular endothelial-derived growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), proliferating cell nuclear antigen (PCNA), extracellular signal-regulated kinase (ERK) 1/2, and nuclear factor kappa B (NF-κB) were detected by immunohistochemical staining. Twenty-four-hour urine protein quantification, serum creatinine, urea nitrogen, and uric acid were collected. Blood stasis syndrome and vessel scores were calculated based on Katafuchi's grade. Intimal thickness/outer vessel diameter (0.2725 ± 0.0932 μm), medial thickness/outer vessel diameter (0.2747 ± 0.1139 μm), and wall thickness/outer vessel diameter (0.6136 ± 0.1120 μm) were the largest in IgAN with arteriopathy group. VEGF (0.35 ± 0.90), MMP-9 (0.38 ± 0.12), PCNA (0.43 ± 0.12), ERK1/2 (0.31 ± 0.11), and NF-κB (0.37 ± 0.14) were the highest in IgAN with arteriopathy group. Intimal thickening of IgAN was moderately positively correlated with VEGF, MMP-9, PCNA, ERK1/2, and NF-κB (0.5 < < 0.8). Medial thickening of IgAN was moderately positively correlated with PCNA and NF-κB (0.5 < < 0.8). Wall thickening of IgAN was lowly positively correlated with VEGF and MMP-9 (0.3 < < 0.5). Blood stasis syndrome score was associated with vessel score in IgAN with arteriopathy ( < 0.05). Blood stasis syndrome score can assess the degree of pathological changes.
探讨免疫球蛋白A肾病（IgAN）血瘀证与动脉病变之间的相关性。使用ImageJ软件测量管壁厚度/血管外径、内膜厚度/血管外径和中膜厚度/血管外径。通过免疫组织化学染色检测血管内皮生长因子（VEGF）、基质金属蛋白酶-9（MMP-9）、增殖细胞核抗原（PCNA）、细胞外信号调节激酶（ERK）1/2和核因子κB（NF-κB）。收集24小时尿蛋白定量、血清肌酐、尿素氮和尿酸。根据片渊分级计算血瘀证和血管评分。内膜厚度/血管外径（0.2725±0.0932μm）、中膜厚度/血管外径（0.2747±0.1139μm）和管壁厚度/血管外径（0.6136±0.1120μm）在伴有动脉病变的IgAN组中最大。VEGF（0.35±0.90）、MMP-9（0.38±0.12）、PCNA（0.43±0.12）、ERK1/2（0.31±0.11）和NF-κB（0.37±0.14）在伴有动脉病变的IgAN组中最高。IgAN的内膜增厚与VEGF、MMP-9、PCNA、ERK1/2和NF-κB呈中度正相关（0.5＜ ＜0.8）。IgAN的中膜增厚与PCNA和NF-κB呈中度正相关（0.5＜ ＜0.8）。IgAN的管壁增厚与VEGF和MMP-9呈低度正相关（0.3＜ ＜0.5）。血瘀证评分与伴有动脉病变的IgAN的血管评分相关（ ＜0.05）。血瘀证评分可评估病理变化程度。
