Pooler J P
Biochim Biophys Acta. 1985 Jan 10;812(1):193-8. doi: 10.1016/0005-2736(85)90538-3.
A kinetic model of colloid osmotic hemolysis for cation-permeable cells has been developed. The model consists of three essential components. The first is a set of flux equations, under the assumption that the membrane potential is equal to the chloride equilibrium potential and that cation fluxes are described by the Goldman flux equation. The second is the osmotic equilibrium model of Freedman and Hoffman that takes into account the non-ideal osmotic behavior of erythrocytes. The third is an empirical relation between hemolysis and cell volume, developed from the lysis behavior in hypoosmotic media. Model simulations are compared with lysis experiments using the antibiotic nystatin to raise cation permeability. The form of the kinetics and inhibition of lysis by sucrose are described well by the model. In additional lysis experiments at different external pH the small pH dependence is accounted for by the model.
已建立了阳离子可通透细胞的胶体渗透溶血动力学模型。该模型由三个基本部分组成。第一部分是一组通量方程，其假设膜电位等于氯离子平衡电位，且阳离子通量由戈德曼通量方程描述。第二部分是弗里德曼和霍夫曼的渗透平衡模型，该模型考虑了红细胞的非理想渗透行为。第三部分是溶血与细胞体积之间的经验关系，它是根据低渗介质中的裂解行为得出的。使用抗生素制霉菌素提高阳离子通透性，将模型模拟结果与裂解实验进行了比较。该模型很好地描述了动力学形式以及蔗糖对裂解的抑制作用。在不同外部pH值下的额外裂解实验中，该模型也解释了较小的pH依赖性。
