Zavodnik I B, Piletskaia T P, Stepuro I I
Biofizika. 1994 Nov-Dec;39(6):1033-9.
Hemolysis of human erythrocytes induced by the high ethanol concentrations involves several stages, which are reflected by the kinetic curves of hemolysis. These stages are of colloid-osmotic nature. Ethanol denaturation of membrane proteins and their subsequent aggregation can be responsible for appearance of defects, pores, in the erythrocyte membrane. About 15 ethanol molecules participate in formation of one pore. Sucrose weakly diffusing through the formed pores inhibits the ethanol-induced hemolysis of erythrocytes. On the contrary, the hyperosmotic contraction of erythrocytes in the NaCl- or KCl-containing medium increases the efficiency of the hemolysis. The maximum stability of the erythrocytes to the ethanol action is at the pH 7.2-7.3.
高浓度乙醇诱导的人红细胞溶血涉及多个阶段，这些阶段由溶血动力学曲线反映出来。这些阶段具有胶体渗透性质。膜蛋白的乙醇变性及其随后的聚集可能导致红细胞膜出现缺陷、孔隙。大约15个乙醇分子参与形成一个孔隙。通过形成的孔隙微弱扩散的蔗糖会抑制乙醇诱导的红细胞溶血。相反，在含NaCl或KCl的介质中红细胞的高渗收缩会提高溶血效率。红细胞对乙醇作用的最大稳定性处于pH 7.2 - 7.3。
