Pooler J P
Biochim Biophys Acta. 1985 Jan 10;812(1):199-205. doi: 10.1016/0005-2736(85)90539-5.
Many of the known features of photohemolysis have been organized in a kinetic model that simulates the lytic time-course in a variety of conditions. The model combines Nernst-Planck flux principles, the osmotic equilibrium model of Freedman and Hoffman, equations relating illumination parameters to ion permeability, and an empirical relation between cell volume and lysis. Model simulations are compared with experiments showing the dependence of lysis kinetics on sensitizer concentration and on the osmotic content of the reaction medium. Additional experiments demonstrate that the inherent osmotic fragility of erythrocytes is not altered by illumination conditions that cause major delayed lysis 23 h later. The successful simulations support the hypothesis that photohemolysis is a colloid osmotic lysis occurring in cells behaving as imperfect osmometers.
光溶血的许多已知特征已被整合到一个动力学模型中，该模型可模拟多种条件下的溶血时间进程。该模型结合了能斯特 - 普朗克通量原理、弗里德曼和霍夫曼的渗透平衡模型、将光照参数与离子通透性相关联的方程，以及细胞体积与裂解之间的经验关系。模型模拟结果与实验进行了比较，实验表明溶血动力学对敏化剂浓度和反应介质的渗透压含量存在依赖性。额外的实验表明，导致23小时后出现严重延迟溶血的光照条件不会改变红细胞固有的渗透脆性。成功的模拟结果支持了光溶血是发生在表现为不完美渗透计的细胞中的胶体渗透裂解这一假设。
