Srinidhi S S Vidya, Murugesan Ambika, Balakrishnan Sekar, Ramesh Maya, Varrshana V, Jacob Mathew
Oral and Maxillofacial Pathology and Oral Microbiology, Vinayaka Mission's Sankarachariyar Dental College, Vinayaka Mission's Research Foundation (Deemed to be University), Salem, Tamil Nadu, India.
J Oral Maxillofac Pathol. 2024 Jul-Sep;28(3):399-402. doi: 10.4103/jomfp.jomfp_542_23. Epub 2024 Oct 15.
Modified Cajal's trichrome stain (MCTS) is a good differential stain that allows one to visibly distinguish between connective tissue and epithelial elements with different tonalities of colour.
Our study aims to evaluate and analyse the effectiveness of oral squamous cell carcinoma (OSCC) using MCTS.
A study was conducted retrospectively with 30 tissue blocks embedded in paraffin from cases of OSCC that have been confirmed by histopathology. Both standard haematoxylin and eosin (H&E) and MCTS were applied to each section. Then all the sections were analysed by two observers for nucleus cytoplasmic intensity, break in the basement membrane, and advancing front of the tumour, muscle, and surrounding stroma. The efficacy of the stain was assessed and was graded as 1, poor; 2, fair; and 3, good based on the staining intensity.
The parameters were graded for H&E and modified Cajal's stain. The results were subjected to the Chi-square test.
The above-mentioned parameters analysed showed a uniformly significant value of 0.001 for comparing modified Cajal's trichrome stain to H&E stain. Measurement of the agreement was done based on Kappa statistics between two observers, and the values for each expression show that there was good agreement between the two for all the parameters.
MCTS can also be used as a diagnostic aid to pathologists for better distinction of cellular components and easier identification, thereby solving difficulties in diagnosis at earlier stages.
改良卡哈尔三色染色法（MCTS）是一种良好的鉴别染色法，能让人通过不同颜色色调清晰区分结缔组织和上皮成分。
本研究旨在评估和分析改良卡哈尔三色染色法（MCTS）用于口腔鳞状细胞癌（OSCC）的有效性。
回顾性研究，选取30例经组织病理学确诊的口腔鳞状细胞癌石蜡包埋组织块。对每个切片同时应用标准苏木精和伊红染色（H&E）及改良卡哈尔三色染色法（MCTS）。然后由两名观察者对所有切片的细胞核细胞质强度、基底膜断裂情况以及肿瘤、肌肉和周围基质的前沿进行分析。根据染色强度评估染色效果并分为1级（差）、2级（中等）和3级（好）。
对H&E染色和改良卡哈尔染色的参数进行分级。结果采用卡方检验。
上述分析参数显示，改良卡哈尔三色染色法与H&E染色相比，差异具有统计学意义（P值均为0.001）。基于两名观察者之间的kappa统计量进行一致性测量；每个指标的值表明，两人在所有参数上均具有良好的一致性。
改良卡哈尔三色染色法（MCTS）也可作为病理学家的诊断辅助手段，以更好地区分细胞成分并便于识别，从而解决早期诊断的困难。
