Ramsay T G, Sheahan J A, Hausman G J, Martin R J
Biol Neonate. 1985;47(1):42-53. doi: 10.1159/000242090.
This experiment was performed to determine if the fetus can influence its placental metabolism. Fetal pigs were decapitated at 45 days of gestation and their placentas compared at 110 days to within litter controls. No histological or histochemical differences were observed. Glycogen levels were elevated in the fetal placenta of decapitated pigs by 107%. Fatty acid synthesis was increased in the fetal placenta of decapitated animals (12.9 +/- 1.5 vs. 3.4 +/- 0.7 nmol acetate units/100 mg/2 h). Fetal decapitation increased fetal placental fatty acid esterification by 43% and oxidation by 125%. The altered lipid metabolism of the fetal placenta was reflected in enzymes associated with substrate utilization (pyruvate kinase and lactate dehydrogenase). Maternal placenta lipogenesis was also altered by fetal manipulation. Leucine conversion to its alpha ketoacid was increased in the fetal placenta of the decapitated pigs (14.8 +/- 1.7 vs. 8.6 +/- 1.4 nmol/100 mg/2 h) indicating amino acid metabolism was affected by this treatment. The ability of an altered fetal endocrinology and metabolism to affect the placental metabolism supports the concept of a fetal influence on placental metabolism.
进行该实验是为了确定胎儿是否会影响其胎盘代谢。妊娠45天时将胎猪断头，并在妊娠110天时将其胎盘与同窝对照胎盘进行比较。未观察到组织学或组织化学差异。断头猪的胎儿胎盘糖原水平升高了107%。断头动物的胎儿胎盘脂肪酸合成增加（12.9±1.5对3.4±0.7 nmol醋酸盐单位/100 mg/2小时）。胎儿断头使胎儿胎盘脂肪酸酯化增加43%，氧化增加125%。胎儿胎盘脂质代谢的改变反映在与底物利用相关的酶（丙酮酸激酶和乳酸脱氢酶）上。母体胎盘脂肪生成也因胎儿操作而改变。断头猪的胎儿胎盘亮氨酸向其α-酮酸的转化增加（14.8±1.7对8.6±1.4 nmol/1oo mg/2小时），表明这种处理影响了氨基酸代谢。胎儿内分泌和代谢的改变影响胎盘代谢的能力支持了胎儿对胎盘代谢有影响这一概念。
