Olivieri W, Wolverton C K, Ruwe P J, White M E, Ramsay T G
Department of Animal Science, Ohio State University, Columbus 43210.
J Nutr. 1990 Oct;120(10):1241-7. doi: 10.1093/jn/120.10.1241.
The present study was conducted to determine whether dietary galactose can be used to improve glycogen and lipid accretion in fetal pigs. Pregnant gilts were fed diets containing either 24% glucose (control) or 24% galactose from d 98 to 110 of gestation. Gilts underwent abdominohysterotomy on d 110 of gestation. Slices of fetal subcutaneous adipose tissue and placenta were examined for metabolic capacity for glucose and for galactose utilization. No effects of maternal diet were evident upon glycogen content or enzyme activity of fetal semitendinosus muscle and liver. Maternal dietary galactose had no direct effects upon placental glucose oxidation or use for lipid synthesis. However, galactose supplementation of the incubation medium caused reductions in glucose oxidation (15%) and total lipid synthesis (24%) by the maternal placenta. Maternal dietary galactose caused an increase in total lipid (50%) and fatty acid synthesis (200%) from glucose in fetal subcutaneous adipose tissue; direct supplementation of galactose to the incubation medium had no effect on these parameters. The results of the present study suggest that feeding galactose to the pregnant gilt does not have direct effects upon placental metabolism or fetal glycogen storage. However, these data indicate that use of galactose in the maternal diet can result in an increase in the utilization of glucose for lipogenesis by fetal adipose tissue in swine. This effect is not a direct effect of galactose because transport across the placenta was not apparent.
本研究旨在确定日粮中的半乳糖是否可用于改善胎猪的糖原和脂质积累。在妊娠第98天至110天，给妊娠母猪饲喂含24%葡萄糖（对照）或24%半乳糖的日粮。在妊娠第110天对母猪进行剖腹取胎术。检查胎儿皮下脂肪组织和胎盘切片对葡萄糖和半乳糖的代谢能力。母体日粮对胎儿半腱肌和肝脏的糖原含量或酶活性没有明显影响。母体日粮中的半乳糖对胎盘葡萄糖氧化或用于脂质合成没有直接影响。然而，在孵育培养基中添加半乳糖会使母体胎盘的葡萄糖氧化（15%）和总脂质合成（24%）减少。母体日粮中的半乳糖使胎儿皮下脂肪组织中葡萄糖的总脂质（50%）和脂肪酸合成（200%）增加；直接在孵育培养基中添加半乳糖对这些参数没有影响。本研究结果表明，给妊娠母猪饲喂半乳糖对胎盘代谢或胎儿糖原储存没有直接影响。然而，这些数据表明，在母体日粮中使用半乳糖可导致猪胎儿脂肪组织中用于脂肪生成的葡萄糖利用率增加。这种作用不是半乳糖的直接作用，因为其跨胎盘转运不明显。
