Pédarrieu Aurélie, Cêtre-Sossah Catherine
CIRAD, UMR ASTRE, Montpellier, France.
ASTRE, University of Montpellier, CIRAD, INRAe, Montpellier, France.
Methods Mol Biol. 2025;2893:37-49. doi: 10.1007/978-1-0716-4338-9_4.
Rift Valley fever (RVF) is one of the main vector-borne zoonotic diseases that affects a wide range of ruminants and humans in Africa and the Arabian Peninsula.Several techniques involving cell culture and molecular biology methods have been developed to diagnose RVF infection. Success partly relies on sending samples to a national or reference laboratory in good conditions and having the capacity to perform the appropriate diagnostic test in the matrix of interest during the period of viremia where high loads of viral particles are present. Conventional and duplex/multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays are currently the most rapid and sensitive molecular tests for the detection and quantification of Rift Valley fever virus (RVFV) during outbreaks. The one-step real-time duplex RT-PCR technique that is detailed in this chapter is based on the L segment of RVFV. Results must be carefully validated and interpreted, taking into account the results of both positive and negative controls.
裂谷热（RVF）是主要的媒介传播人畜共患病之一，在非洲和阿拉伯半岛影响着广泛的反刍动物和人类。已经开发了几种涉及细胞培养和分子生物学方法的技术来诊断裂谷热感染。成功部分依赖于在良好条件下将样本送往国家或参考实验室，并具备在病毒血症期间（存在高负荷病毒颗粒）在所关注的基质中进行适当诊断测试的能力。常规和双重/多重实时逆转录聚合酶链反应（RT-PCR）检测目前是疫情期间检测和定量裂谷热病毒（RVFV）最快速、灵敏的分子检测方法。本章详细介绍的一步法实时双重RT-PCR技术基于裂谷热病毒的L片段。必须仔细验证和解释结果，同时考虑阳性和阴性对照的结果。
