McElroy Anita K
Pediatric Infectious Disease and Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Methods Mol Biol. 2025;2893:137-150. doi: 10.1007/978-1-0716-4338-9_11.
Characterization and quantitation of T cell responses following infection and/or vaccination can provide insight into mechanisms of host cell immunity that provide resolution of acute infection or protection from future infection or disease. While these types of studies are very advanced for viruses such as HIV, influenza, and SARS-CoV-2, they are less well developed for most of the Bunyaviruses. Cytotoxic CD8T cells are especially relevant in the context of viral infections since they recognize virus-infected cells via interaction of the T cell receptor with virally derived peptides presented in the context of MHCI. CD4T follicular cells are especially important for augmenting the antiviral antibody response. This chapter provides methods for characterizing T cell responses post infection/vaccination in both mice and humans as well as several methods for quantifying virus-specific T cells with the goal of arming bunyavirus researchers with the tools needed to move the field forward.
感染和/或接种疫苗后T细胞反应的表征和定量分析可以深入了解宿主细胞免疫机制，这些机制能够解决急性感染或预防未来的感染或疾病。虽然针对HIV、流感和SARS-CoV-2等病毒的这类研究已经非常先进，但对于大多数布尼亚病毒来说，它们的发展还不够完善。细胞毒性CD8T细胞在病毒感染的情况下尤其重要，因为它们通过T细胞受体与MHC I背景下呈递的病毒衍生肽相互作用来识别病毒感染的细胞。CD4T滤泡细胞对于增强抗病毒抗体反应尤为重要。本章提供了在小鼠和人类中表征感染/接种疫苗后T细胞反应的方法，以及几种定量病毒特异性T细胞的方法，目的是为布尼亚病毒研究人员提供推动该领域前进所需的工具。
