Elfadadny Medhat R, Furukawa Miho, Nozaki Reiko, Kawato Satoshi, Koiwai Keiichiro, Kondo Hidehiro, Hirono Ikuo
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan.
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Damanhur University, Damanhur, Egypt.
Mol Biol Rep. 2024 Dec 13;52(1):48. doi: 10.1007/s11033-024-10147-x.
Shrimp gill health is an indicator of their overall health and environmental stress, as gills are in direct contact with the external environment. Organic selenium (OS), known for its high bioavailability and antioxidant properties, potentially enhances shrimp growth, survival, and immune responses. This study investigated the physiological features through comprehensive gill gene expression profiling and growth parameters by incorporating OS into Penaeus vannamei diets.
Two OS-containing products, AlkoSel (AS) and SelPlex (SL), were supplied to the diet of P. vannamei (0.90 ± 0.05 g body weight, 4.54 ± 0.5 cm length) at a dose of 0.4 mg Se kg ration in the final feed. Three groups were organized: an AS-fed group, an SL-fed group, and a control (C) group without additives. After a one-month feeding trial, performance indices (PI) were assessed and RNA-Seq analysis was conducted. The PI revealed that both OS products had similar growth-stimulating effects, with no significant differences between the two groups. However, both groups showed significant improvement compared to the C-group. The P. vannamei gill transcriptome was strongly impacted by the two OS products, with several KEGG pathways implicated in their metabolic mechanisms. Pathways related to energy metabolism, carbohydrate metabolism, lipid metabolism, non-specific immunity, and antioxidative responses were significant in regulating the addition of AS and SL to P. vannamei diets.
While SL had some higher additive effects on the gene expression profile than AS, OS from either product is an essential micronutrient that promotes growth, normal metabolic activities, and good gill health in P. vannamei during the early feeding period.
虾鳃健康是其整体健康和环境应激的指标，因为鳃直接与外部环境接触。有机硒（OS）以其高生物利用度和抗氧化特性而闻名，可能会促进虾的生长、存活和免疫反应。本研究通过将有机硒纳入凡纳滨对虾饲料中，通过全面的鳃基因表达谱分析和生长参数来研究其生理特征。
将两种含有机硒的产品，即AlkoSel（AS）和SelPlex（SL），以0.4毫克硒/千克日粮的剂量添加到凡纳滨对虾（体重0.90±0.05克，体长4.54±0.5厘米）的饲料中。实验分为三组：AS投喂组、SL投喂组和无添加剂的对照组（C组）。经过为期一个月的投喂试验，评估了生长性能指标（PI）并进行了RNA测序分析。PI结果显示，两种有机硒产品具有相似的促生长作用，两组之间无显著差异。然而，与C组相比，两组均表现出显著改善。两种有机硒产品对凡纳滨对虾鳃转录组有强烈影响，其代谢机制涉及多个KEGG通路。与能量代谢、碳水化合物代谢、脂质代谢、非特异性免疫和抗氧化反应相关的通路在调节AS和SL添加到凡纳滨对虾饲料中具有重要作用。
虽然SL对基因表达谱的添加效应比AS略高，但两种产品中的有机硒都是必需的微量营养素，在早期投喂阶段可促进凡纳滨对虾的生长、正常代谢活动和良好的鳃健康。
