Guzman John Paul Matthew Domingo, Nozaki Reiko, Aoki Mikio, Kuwahara Hiroshi, Mikata Kazuki, Koiwai Keiichiro, Kondo Hidehiro, Hirono Ikuo
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo, 108-8477, Japan; Environment and Biotechnology Division, Industrial Technology Development Institute, Department of Science and Technology, Philippines.
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo, 108-8477, Japan.
Fish Shellfish Immunol. 2025 Feb;157:110091. doi: 10.1016/j.fsi.2024.110091. Epub 2024 Dec 12.
The lack of a classical adaptive immunity renders the development of disease control and prevention measures in shrimp challenging. In this study, the concept of trained immunity was exploited in the development of a feed supplement. Penaeus vannamei shrimp was fed with feed supplemented with freeze-dried whole culture of Lactiplantibacillus plantarum (FD-LAB) for 15 days. RNA sequencing using Illumina platform was performed on the gill and stomach tissues collected at specific time points during the feeding period (0 day, 8th day, 15th day). Differentially-expressed genes (DEGs) previously reported to have innate immunity- and immune memory-related functions were selected for validation. Additionally, the differential expression of putatively immune-related circular RNAs (DECs) were also explored as these noncoding regulatory RNAs may also influence host immunity. Challenge tests with either the acute hepatopancreatic necrosis disease-causing strain Vibrio parahaemolyticus D6 or White Spot Syndrome Virus (WSSV) were conducted. Transcriptome analyses showed that FD-LAB supplementation resulted to DEGs and DECs related to pathogen recognition, antimicrobial peptides, transcription regulation, and immune memory. Challenge tests performed immediately after 15 days and 8 days of feeding showed protection on P. vannamei by FD-LAB against bacterial and viral pathogens. Increase in survival rates were also observed upon challenge with both pathogens 7 days and 14 days after last intake of FD-LAB, indicating trained immunity in shrimp. Our study highlighted the effects of FD-LAB on the innate immunity and immune memory of P. vannamei against bacterial and viral pathogens. These findings emphasize the possibility of immunostimulants inducing lasting enhanced immunity against infections despite the lack of a classical adaptive immunity in shrimp.
缺乏经典的适应性免疫使得开发虾类疾病控制和预防措施具有挑战性。在本研究中，在开发一种饲料添加剂时利用了训练免疫的概念。用添加了植物乳杆菌冻干全培养物（FD-LAB）的饲料喂养凡纳滨对虾15天。在喂养期间的特定时间点（第0天、第8天、第15天）收集鳃和胃组织，使用Illumina平台进行RNA测序。选择先前报道具有先天免疫和免疫记忆相关功能的差异表达基因（DEG）进行验证。此外，还探索了假定的免疫相关环状RNA（DEC）的差异表达，因为这些非编码调节RNA也可能影响宿主免疫力。用急性肝胰腺坏死病致病菌株副溶血性弧菌D6或白斑综合征病毒（WSSV）进行攻毒试验。转录组分析表明，添加FD-LAB导致了与病原体识别、抗菌肽、转录调控和免疫记忆相关的DEG和DEC。在喂养15天和8天后立即进行的攻毒试验表明，FD-LAB对凡纳滨对虾有针对细菌和病毒病原体的保护作用。在最后一次摄入FD-LAB后7天和14天用两种病原体攻毒时也观察到存活率增加，表明虾具有训练免疫。我们的研究突出了FD-LAB对凡纳滨对虾针对细菌和病毒病原体的先天免疫和免疫记忆的影响。这些发现强调了尽管虾缺乏经典的适应性免疫，但免疫刺激剂仍有可能诱导对感染的持久增强免疫力。
