Sakura H, Hamada Y, Tsuruta S, Okamoto K, Nakamura S
Cancer. 1985 Mar 1;55(5):1001-6. doi: 10.1002/1097-0142(19850301)55:5<1001::aid-cncr2820550514>3.0.co;2-9.
A primary ovarian carcinoid composed of both trabecular and strumal types was studied by histochemical, immunocytochemical, and biochemical techniques. High contents of glucagon, secretin, and calcitonin were demonstrated in the tumor homogenate. All of the tumor cells, irrespective of histologic type, showed properties of argyrophilia and neurosecretory granules on electron microscopy. Glucagon-producing cells were positive in trabecular carcinoid by immunoperoxidase techniques. Bio-Gel P10 gel filtration showed that the molecular weight of major immunoreactive glucagon in tumor was 20,000. It migrated faster than true glucagon after polyacrylamide gel electrophoresis. No clinical symptoms of glucagonoma developed.
采用组织化学、免疫细胞化学和生物化学技术对一例同时含有小梁型和甲状腺肿型成分的原发性卵巢类癌进行了研究。肿瘤匀浆中显示出高含量的胰高血糖素、促胰液素和降钙素。电镜下，所有肿瘤细胞，无论组织学类型如何，均表现出嗜银性和神经分泌颗粒的特性。通过免疫过氧化物酶技术，产生胰高血糖素的细胞在小梁型类癌中呈阳性。生物凝胶P10凝胶过滤显示肿瘤中主要免疫反应性胰高血糖素的分子量为20,000。聚丙烯酰胺凝胶电泳后，其迁移速度比真正的胰高血糖素快。未出现胰高血糖素瘤的临床症状。
