Barimah Alberta Osei, Tai Shengmei, Sun Chengdong, Ding Tao, Peng Chifang, Mao Minxin, Wang Zhouping, Agyekum Akwasi Akomeah
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Animal, Plant and Food Inspection Center, Nanjing Customs, Nanjing, 210019, China.
Talanta. 2025 Apr 1;285:127369. doi: 10.1016/j.talanta.2024.127369. Epub 2024 Dec 12.
Antibiotic contaminants in food are associated with detrimental health issues, and thus, the design of rapid and reliable detection methods to monitor them is in high demand. Although colorimetric aptamer sensing methods can achieve accurate and quick visual read-out analysis, they still have morphological defects that affect the detection efficiency and stability of the aptasensor. Hence, a simple and highly selective iron oxide/graphene oxide (FeO/GO) nanospheres was developed and modified with an aptamer as nanozymes for colorimetric detection of tobramycin (Tob). Due to the electrostatic and π-π stacking interaction between the aptamer and the spherical FeO/GOs nanozyme, the catalytic activity of the nanozyme was enhanced by the robust affinity between the aptamer and the 3,3',5,5'-tetramethylbenzidine (TMB) substrate. However, the presence of Tob could obstruct the catalytic activity through the specific binding of aptamer to Tob and the detachment of aptamer from the surface of the FeO/GO, causing a low catalytic activity. Based on this strategy, a visual and quantitative detection for Tob was achieved with a detection limit of 70.7 nM. The proposed aptasensor demonstrated high selectivity, ease of operation, and potential application in food samples with satisfactory recoveries of 98.4-108.5 %. Therefore, this proposed method has great prospects for monitoring food safety and could be applied for sensing varied antibiotics by just switching the target-specific aptamer.
食品中的抗生素污染物与有害健康问题相关，因此，对快速可靠的检测方法进行设计以监测这些污染物的需求很高。尽管比色适体传感方法能够实现准确且快速的视觉读出分析，但它们仍存在形态缺陷，影响适体传感器的检测效率和稳定性。因此，开发了一种简单且具有高选择性的氧化铁/氧化石墨烯（FeO/GO）纳米球，并用适体作为纳米酶进行修饰，用于比色检测妥布霉素（Tob）。由于适体与球形FeO/GOs纳米酶之间存在静电和π-π堆积相互作用，适体与3,3',5,5'-四甲基联苯胺（TMB）底物之间的强亲和力增强了纳米酶的催化活性。然而，Tob的存在会通过适体与Tob的特异性结合以及适体从FeO/GO表面的脱离来阻碍催化活性，导致催化活性较低。基于此策略，实现了对Tob的可视化定量检测，检测限为70.7 nM。所提出的适体传感器具有高选择性、操作简便，在食品样品中的回收率为98.4-108.5%，具有潜在应用价值。因此，该方法在食品安全监测方面具有广阔前景，只需更换目标特异性适体即可用于检测多种抗生素。
