Danes B S
Cancer Genet Cytogenet. 1985 Jan 15;14(3-4):285-91. doi: 10.1016/0165-4608(85)90194-3.
The relative importance of the genome and environmental factors has not been established in pancreatic carcinogenesis. The identification of in vitro expressions of cancer genes should enhance such clarification. Hyperdiploidy, with the exception of a low incidence (0%-7%) of tetraploidy, has rarely been observed in dermal monolayer cultures from normal individuals without a family history of solid tumors. Hyperdiploidy (incidence over 1% in a culture) was not observed in dermal fibroblast monolayer cultures derived from 143 clinical normals (ages 18-80 years). In vitro hyperdiploidy (7%-14%), with a normal occurrence of tetraploidy (0%-3%), was observed in 6 of 28 patients with acinar adenocarcinoma of the body of the pancreas. No in vitro hyperdiploidy was observed in the remaining pancreatic cancer patients or in the 25 spouses with negative family cancer histories studied. As hyperdiploidy with a normal occurrence of tetraploidy has been previously reported [1] in a familial pancreatic cancer cluster, this chromosomal alteration was considered to be an in vitro expression of genetic predisposition for pancreatic cancer in approximately 20% of this small (28 patients) sample. In vitro studies on a significantly larger patient sample will be required to determine its incidence in a patient group with acinar adenocarcinoma of the body of the pancreas.
在胰腺癌发生过程中，基因组和环境因素的相对重要性尚未明确。癌症基因体外表达的鉴定应有助于此类问题的阐明。除了四倍体发生率较低（0%-7%）外，在无实体瘤家族史的正常个体的皮肤单层培养物中很少观察到超二倍体。在来自143名临床正常个体（年龄18-80岁）的皮肤成纤维细胞单层培养物中未观察到超二倍体（培养物中发生率超过1%）。在28例胰体腺泡腺癌患者中的6例中观察到体外超二倍体（7%-14%），四倍体正常发生率为（0%-3%）。在其余胰腺癌患者或研究的25名无家族癌症病史的配偶中未观察到体外超二倍体。由于先前在一个家族性胰腺癌聚集病例中报告过伴有正常四倍体发生率的超二倍体[1]，在这个小样本（28例患者）中，这种染色体改变被认为是胰腺癌遗传易感性的一种体外表现，约占20%。需要对更大的患者样本进行体外研究，以确定其在胰体腺泡腺癌患者群体中的发生率。
