Perucchini Mariasofia, Vigani Barbara, Ruggeri Marco, Pellegrini Angelica, Pietrocola Giampiero, Varacca Giada, Bettini Ruggero, Milanese Chiara, Sandri Giuseppina, Rossi Silvia
Department of Drug Sciences, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy.
Department of Drug Sciences, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy.
Int J Biol Macromol. 2025 Feb;288:138674. doi: 10.1016/j.ijbiomac.2024.138674. Epub 2024 Dec 11.
Respiratory tract infections (RTIs) represent a significant global health issue, particularly for vulnerable population, such as children, the elderly, or patients with immunosuppression. In this context, the aim of the present work was the development of Chitosan/Hydrolyzed Collagen-based microparticles (Mps) as a pulmonary drug delivery system (PDDS) for the treatment of RTIs. Mps were produced via spray-drying and composed of chitosan (Cs), one of the most widely used polysaccharides in PDDS, and hydrolyzed collagen (HC), another promising material for the development of PDDS that has not yet been fully explored. The formation of an interaction product between Cs and HC occurred during the spray-drying process and was confirmed by infrared spectroscopy and thermal analysis. Mps were characterized in terms of morphology, particle size, zeta potential, aerodynamic performance, swelling behavior and biodegradation profile in simulated lung fluid. Mps biocompatibility was also assessed on adenocarcinomic human alveolar basal epithelial (A549) cells. Finally, Mps were characterized in vitro for antibacterial properties and their ability to inhibit bacterial adhesion to S. aureus and P. aeruginosa. An enhanced antibacterial effect was observed for Mps with respect to the pristine materials (Cs and HC) and their physical mixture. Moreover, Mps were also able to inhibit bacteria adhesion to epithelial cells.
呼吸道感染（RTIs）是一个重大的全球健康问题，尤其对于弱势群体，如儿童、老年人或免疫抑制患者。在此背景下，本研究的目的是开发基于壳聚糖/水解胶原蛋白的微粒（Mps）作为用于治疗呼吸道感染的肺部给药系统（PDDS）。Mps通过喷雾干燥制备，由壳聚糖（Cs）和水解胶原蛋白（HC）组成，壳聚糖是PDDS中使用最广泛的多糖之一，而水解胶原蛋白是一种尚未得到充分探索的、开发PDDS的有前景的材料。Cs和HC之间相互作用产物的形成发生在喷雾干燥过程中，并通过红外光谱和热分析得到证实。对Mps的形态、粒径、zeta电位、空气动力学性能、溶胀行为以及在模拟肺液中的生物降解特性进行了表征。还在人肺泡基底上皮腺癌（A549）细胞上评估了Mps的生物相容性。最后，对Mps的抗菌性能及其抑制细菌黏附金黄色葡萄球菌和铜绿假单胞菌的能力进行了体外表征。与原始材料（Cs和HC）及其物理混合物相比，观察到Mps具有增强的抗菌效果。此外，Mps还能够抑制细菌黏附上皮细胞。
