Batista Miguel P, Pimenta Margarida, Fernández Naiara, Duarte Ana Rita C, Bronze Maria do Rosário, Marto Joana, Gaspar Frédéric Bustos
iBET-Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal.
LAQV-REQUIMTE, Associated Laboratory for Green Chemistry-Network of Chemical and Technology, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Caparica, Portugal.
Pharmaceutics. 2025 May 6;17(5):618. doi: 10.3390/pharmaceutics17050618.
Recent studies highlight the excellent wound-healing properties of collagen and chitosan materials. Combining these polymers with a bioactive compound could enhance their effectiveness as next-generation wound dressings. Hydroxytyrosol (HT), an antioxidant derived from olive oil, may aid wound healing due to its anti-inflammatory, antimicrobial, and angiogenesis-stimulating properties, making it a beneficial addition to collagen-chitosan dressings. It could be a beneficial addition to collagen-chitosan dressings, thus improving their therapeutic effects. This study screens the potential of collagen-chitosan composites with HT for wound-healing applications and assesses the influence of the compound's incorporation on the materials' properties. The material production involved incorporating chitosan and HT into a marine collagen extract. The resulting collagen-chitosan-HT material was obtained through freeze-drying. Prototype dressing characterization included morphology by scanning electron microscopy, solid and hydrated state by textural and rheological studies, and in vitro HT release studies. The materials' cytocompatibility screening was assessed using a mouse fibroblast cell line, and the antibacterial activity was evaluated against microorganisms commonly implicated in wound infections. Burst strength, viscosity, frequency sweep test, tackiness, and adhesion results indicate that chitosan contributes to the material's mechanical robustness by maintaining a high viscosity and preserving the material's gel structure. The in vitro release studies suggest an HT-controlled release profile with a maximum release (70%) achieved after 10 h. Biological experiments confirmed the materials' cytocompatibility with skin cells and very promising antibacterial efficacy against and . In conclusion, HT was successfully incorporated into a collagen-chitosan matrix, enhancing the therapeutic prospect of the resultant material. The collagen-chitosan-HT composite presents a promising potential as an advanced wound-healing material.
最近的研究突出了胶原蛋白和壳聚糖材料出色的伤口愈合特性。将这些聚合物与生物活性化合物结合，可以提高它们作为下一代伤口敷料的有效性。羟基酪醇（HT）是一种从橄榄油中提取的抗氧化剂，因其具有抗炎、抗菌和刺激血管生成的特性，可能有助于伤口愈合，使其成为胶原蛋白-壳聚糖敷料的有益添加物。它可能是胶原蛋白-壳聚糖敷料的有益添加物，从而提高其治疗效果。本研究筛选了含HT的胶原蛋白-壳聚糖复合材料在伤口愈合应用中的潜力，并评估了该化合物的加入对材料性能的影响。材料制备过程包括将壳聚糖和HT加入到海洋胶原蛋白提取物中。通过冷冻干燥得到最终的胶原蛋白-壳聚糖-HT材料。原型敷料的表征包括通过扫描电子显微镜观察形态、通过质地和流变学研究分析固态和水合状态，以及进行体外HT释放研究。使用小鼠成纤维细胞系评估材料的细胞相容性，并针对通常与伤口感染有关的微生物评估抗菌活性。破裂强度、粘度、频率扫描测试、粘性和粘附结果表明，壳聚糖通过保持高粘度和维持材料的凝胶结构，有助于提高材料的机械强度。体外释放研究表明HT具有可控的释放曲线，10小时后达到最大释放量（70%）。生物学实验证实了材料与皮肤细胞的细胞相容性以及对……和……非常有前景的抗菌效果。总之，HT成功地掺入到胶原蛋白-壳聚糖基质中，提高了所得材料的治疗前景。胶原蛋白-壳聚糖-HT复合材料作为一种先进的伤口愈合材料具有广阔的潜力。
